福建农业学报2026，Vol.41Issue(6)：704-713,10.DOI:10.19303/j.issn.1008-0384.2026.06.008
凤凰木重伞灵芝菌根腐病生防菌株的筛选及其发酵优化
Isolation and Culture of Bacteria Antagonistic to Ganoderma multipileum in Delonix regia
摘要
Abstract
[Objective]Bacteria antagonistic to Ganoderma multipileum that causes root rot on Delonix regia were identified,and their culture conditions optimized for biocontrol of the increasingly spread disease on the landscape ornamental trees.[Method]Using dual culture method,anti-G.multipileum microbes were isolated with taxonomic identities determined by morphological and molecular means.Conditions for culturing the isolates were optimized.In vitro control efficacy on the pathogen was examined by a pot experiment.[Result]The consortium of Strains FH-3 and FH-7,which were identified as Pseudomonas aeruginosa and Bacillus licheniformis,respectively,exhibited an inhibition rate of 72.80%against G.multipileum.In single-factor and orthogonal experiments,the culture of the mixed consortium was optimized to have a medium containing 3%of sucrose,0.5%of ammonium sulfate,1%of beef extract,and 2%of potassium chloride with 3%of inoculum to propagate under 32℃,pH 7,and constant stirring at 220 r·min-1 for 24 h.As a result,the inhibition rate on G.multipileum was raised to 88.70%.[Conclusion]The mixed FH-3 and FH-7 significantly inhibited the growth of G.multipileum,indicating a potential of developing the strains for controlling the root rot disease on D.regia.关键词
凤凰木/重伞灵芝/根腐病/生防菌Key words
Delonix regia/Ganoderma multipileum/root rot/biocontrol bacteria分类
农业科技引用本文复制引用
覃艳丽,王年,马卓,左舒翟,李雅颖,任引..凤凰木重伞灵芝菌根腐病生防菌株的筛选及其发酵优化[J].福建农业学报,2026,41(6):704-713,10.基金项目
福建省科技计划STS项目(2023T3021) （2023T3021）
厦门市科学技术局揭榜挂帅项目(3502Z20251016) （3502Z20251016）
国家重点研发计划项目(2022YFF1303001) （2022YFF1303001）
