福建农业学报2026，Vol.41Issue(6)：723-730,8.DOI:10.19303/j.issn.1008-0384.2026.06.010
都匀市茶树叶斑病病原鉴定及药剂敏感性测定
Pathogen Identification and Fungicide Susceptibility of Tea Leaf Spot Disease at Duyun
摘要
Abstract
[Objective]Pathogen of the tea leaf spot disease occurred at Duyun was identified and effective fungicides for field control determined.[Method]Pathogenic microbes were isolated and purified using the tissue isolation method.Pathogenicity of the isolates was verified according to Koch's protocol.Positive pathogen was identified according to morphological observation and phylogenetic analysis with concatenated multi-gene sequences including nuclear ribosomal RNA internal transcribed spacer(ITS),translation elongation factor 1-α(TEF1-α),and β-tubulin(TUB2).Susceptibility of the isolate to various germicides was evaluated using the mycelial growth rate method.[Result]The pathogen that caused the leaf spot disease on tea plants at Duyun was identified as Botryosphaeria dothidea.It was the first reported case in Guizhou Province.Of various fungicides,the 50%carbendazim wettable powder showed the strongest inhibitory activity against the fungus,with a median effective concentration(EC50)of 0.16 mg·L-1.The 40%difenoconazole suspension concentrate and 45%prochloraz emulsion in water had EC50 of 3.84 mg·L-1 and 5.00 mg·L-1,respectively.The biological agents,specifically the 0.5%chitosan aqueous solution and 25%oligosaccharide·ethylicin microemulsion,also exhibited substantial inhibitory activities,with EC50 of 17.68 mg·L-1 and 26.01 mg·L-1,respectively.[Conclusion]B.dothidea was identified as the pathogen of the leaf spot disease on tea plants at Duyun.Chemical fungicides,e.g.,carbendazim,difenoconazole,and prochloraz,were recommended for controlling severe outbreaks and the bio-fungicides,such as chitosan or oligosaccharide·ethylicin,for mild cases detected at early stages.关键词
茶树/叶斑病/葡萄座腔菌/病原鉴定/药剂敏感性Key words
Camellia sinensis/leaf spot/Botryosphaeria dothidea/pathogen identification/fungicide susceptibility分类
农业科技引用本文复制引用
王兴娥,龙仕蓉,韦祥缝,万前丹,张佳惠,张丹丹,陈欣怡,黄太凤,赵永田..都匀市茶树叶斑病病原鉴定及药剂敏感性测定[J].福建农业学报,2026,41(6):723-730,8.基金项目
国家自然科学基金项目(32360652) （32360652）
贵州省科技计划项目(黔科合基础MS[2025]341) （黔科合基础MS[2025]341）
黔南民族师范学院高层次人才科研专项(2021qnsyrc07、qnsyrc202309) （2021qnsyrc07、qnsyrc202309）
黔南民族师范学院教育质量提升工程项目(2021xjg018) （2021xjg018）
贵州省大学生创新创业训练计划项目(S2025106701639、S2025106701647) （S2025106701639、S2025106701647）
