分析化学2026，Vol.54Issue(8)：1389-1399,中插28-中插30,14.DOI:10.19756/j.issn.0253-3820.251346
基于重组酶聚合酶扩增-CRISPR策略的电化学生物传感器用于海月水母环境DNA的快速检测
An Electrochemical Biosensor Based on Recombinase Polymerase Amplification-CRISPR Strategy for Rapid Detection of Aurelia spp.Environmental DNA
摘要
Abstract
The outbreak of the Aureliaspp.can cause potential safety risks by blocking the cooling water intake of nuclear power plants.However,current biological monitoring methods are unable to meet the requirements of rapid monitoring and early warning.In this study,an electrochemical biosensor based on recombinase polymerase amplification(RPA)and clustered regularly interspaced short palindromic repeats(CRISPR)was developed for rapid detection of Aurelia spp.environmental DNA(eDNA).The results demonstrated that the biosensor enabled signal readout within 45 min and exhibited excellent analytical performance,including a wide detection range(10‒1010 copy/μL),low limit of detection(1.89 copy/μL)and limit of quantitation(5.62 copy/μL).The comparison of the testing results of aquarium samples provided by the biosensor and real-time quantitative PCR confirmed the accuracy of the biosensor,while Bland-Altman analysis further confirmed the agreement between the two methods.In actual sample analysis,the detection results obtained by the biosensor were consistent with those from ecological surveys.The developed biosensor demonstrated promising potential for application in monitoring Aurelia spp..关键词
海月水母/环境DNA/重组酶聚合酶扩增/规律间隔成簇短回文重复序列/电化学生物传感器Key words
Aurelia spp./Environmental DNA/Recombinase polymerase amplification/Clustered regularly interspaced short palindromic repeats/Electrochemical biosensor引用本文复制引用
叶长睿,张朝鑫,吴可欣,张曼,王少鹏,张翠忠,王丽伟..基于重组酶聚合酶扩增-CRISPR策略的电化学生物传感器用于海月水母环境DNA的快速检测[J].分析化学,2026,54(8):1389-1399,中插28-中插30,14.基金项目
国家自然科学基金项目(No.22564003)、广西八桂青年拔尖人才项目(No.GXR-2BGQ2525028)和广西民族师范学院高层次人才项目(No.2025XBNGCC27)资助. Supported by the National Natural Science Foundation of China(No.22564003),the Guangxi Bagui Talent Project(No.GXR-2BGQ2525028)and the High-Level Talent Project of Guangxi Minzu Normal University(No.2025XBNGCC27). （No.22564003）
