赣南医科大学学报2026，Vol.46Issue(8)：695-700,6.DOI:10.3969/j.issn.2097-7174.2026.08.001
葛根素通过激活PI3K/Akt信号通路减轻2型糖尿病大鼠心肌缺血再灌注损伤
Puerarin attenuates cardiac ischemia/reperfusion injury in type 2 diabetic rats by activating the PI3K/Akt signaling pathway
摘要
Abstract
Objective:To investigate the protective effect of puerarin(Pue)against cardiac ischemia/reperfusion(I/R)injury in type 2 diabetic(T2DM)rats and to explore its regulatory mechanism on the PI3K/Akt signaling pathway.Methods:A T2DM male SD rat model was established by selecting 30 sixteen-week-old T2DM male SD rats,which were randomly divided into three groups:sham group(Sham),ischemia-reperfusion group(I/R),and puerarin pretreatment group(I/R+Pue),with 10 rats in each group.The I/R+Pue group received intraperitoneal injections of puerarin at 50 mg/(kg·d)for three consecutive days,while the Sham and I/R groups received an equal volume of saline instead.Twenty-four hours after the final drug administration,myocardial I/R model was established by ligating the left anterior descending coronary artery for 30 minutes followed by 2 hours of reperfusion.Fasting blood glucose(FBG)levels were monitored one day before puerarin administration and three days after treatment.Echocardiography was used to measure left ventricular ejection fraction(EF).Serum levels of myocardial injury markers(CK,LDH,cTnI)and liver/kidney function indicators were assessed by ELISA.Myocardial pathological damage and apoptosis were evaluated using hematoxylin-eosin(HE)staining and TUNEL assay,respectively.Western blot analysis was performed to detect protein expression of PI3K and p-Akt.Results:Compared with the Sham group,there was no statistically significant difference in serum FBG,ALT,AST,BUN,and Cr levels between the I/R group before drug administration and 3 days after administration(P>0.05),while CK,LDH,and cTnI levels were significantly elevated,showing statistical significance(P<0.05).Compared with the I/R group,the I/R+Pue group showed no significant differences in serum ALT,AST,BUN,and Cr levels(P>0.05),but FBG,CK,LDH,and cTnI levels were significantly reduced 3 days after administration,with statistical significance(P<0.05).HE staining revealed that myocardial cells in the Sham group had regular morphology,orderly arrangement,and clear striations,without cellular edema or inflammatory infiltration.In contrast,myocardial cells in the I/R group exhibited irregular shapes,disordered arrangement,loss of striations in some areas,obvious inflammatory cell infiltration,cytoplasmic edema,and degeneration or necrosis in some cells.Compared with the I/R group,the I/R+Pue group showed significantly reduced myocardial tissue damage,less inflammatory cell infiltration,and relatively intact myocardial cell structure.TUNEL staining showed minimal apoptosis in the Sham group,whereas numerous TUNEL-positive cells(green fluorescence)were observed in the I/R group.The proportion of TUNEL-positive cells was significantly higher in the I/R group compared with the Sham group(P<0.05),and significantly lower in the I/R+Pue group compared with the I/R group(P<0.05).Western blot results indicated that,compared with the Sham group,PI3K and p-Akt levels in the I/R group were significantly decreased(P<0.05),whereas in the I/R+Pue group,these levels were significantly increased compared with the I/R group(P<0.05).Conclusion:Puerarin can significantly attenuate myocardial I/R injury and improve cardiac contractile function in T2DM rats,and the underlying mechanism may involve activation of the PI3K/Akt signaling pathway and inhibition of cardiomyocyte apoptosis.关键词
葛根素/缺血再灌注损伤/2型糖尿病/PI3K/Akt信号通路/心肌保护Key words
Puerarin/Ischemia/reperfusion injury/Type 2 diabetes mellitus/PI3K/Akt signaling pathway/Myocardial protection分类
医药卫生引用本文复制引用
李兵达,程欣,周山庚,木沙江·艾尼,贺应林,涂赟..葛根素通过激活PI3K/Akt信号通路减轻2型糖尿病大鼠心肌缺血再灌注损伤[J].赣南医科大学学报,2026,46(8):695-700,6.基金项目
江西省中医药管理局科技计划项目(2021B123) （2021B123）
