寒旱农业科学2026，Vol.5Issue(9)：848-855,8.DOI:10.3969/j.issn.2097-2172.2026.09.010
一株解磷印度假单胞菌YWIP10的筛选鉴定及其耐盐碱与解磷特性
Screening,Identification,Saline-alkali Tolerance and Phosphate-solubilizing Characteristics of a Phosphate-solubilizing Pseudomonas Strain YWIP10
摘要
Abstract
Phosphorus availability is low and environmental stresses are severe in saline-alkali soils.This study aimed to iso-late and screen phosphate-solubilizing and saline-alkali tolerant strains from saline-alkali soils in Xinjiang,determine their taxonom-ic classification,saline-alkali tolerance characteristics,and phosphate-solubilizing mechanisms,so as to provide high-quality micro-bial resources and theoretical support for developing microbial fertilizers for saline-alkali soil improvement.Phosphate-solubilizing strains were isolated and screened from saline-alkali soils in Xinjiang using inorganic phosphorus medium.The strain was identified via morphological observation,Gram staining,scanning electron microscopy(SEM),and 16S rRNA gene sequencing.Phosphate-solu-bilization dynamics and stress tolerance were evaluated,and the types and concentrations of organic acids were determined by high-performance liquid chromatography(HPLC)to elucidate the underlying phosphate-solubilizing mechanisms.A stress-tolerant phos-phate-solubilizing strain designated YWIP10 was obtained,which was identified as Pseudomonas indica.In inorganic phosphate medi-um,strain YWIP10 exhibited the highest phosphate-solubilizing activity after 7 d of incubation,with the soluble phosphorus concen-tration reaching 266.01 μg/mL.Meanwhile,the pH of the fermentation broth decreased from an initial value of 5.1 to 4.7,showing a distinct acidification effect.Strain YWIP10 retained phosphate-solubilizing activity at NaCl concentrations of≤40 g/kg and within a pH range of 7.0 to 9.0.A total of 9 organic acids were detected in the fermentation broth of strain YWIP10.Compared with the corre-sponding medium without tricalcium phosphate,the acetic acid concentration in the inorganic phosphate medium showed the greatest increase,reaching 250.37%,while maleic acid,lactic acid,tartaric acid,and citric acid increased by 66.67%,39.85%,9.69%,and 4.37%,respectively,leading to a total increase of 53.79% in the total organic acid content.It is inferred that the strain promotes the dissolution of insoluble phosphorus by secreting multiple organic acids,which reduce the environmental pH value and chelate metal ions.Pseudomonas indica YWIP10 exhibits stable phosphate-solubilizing capacity and excellent saline-alkali tolerance.Its phos-phate-solubilizing mechanism is closely associated with the synergistic secretion of multiple organic acids.This strain can serve as a microbial resource for saline-alkali soil improvement and the development of microbial fertilizers.关键词
解磷菌株/印度假单胞菌/筛选鉴定/耐盐碱/有机酸/解磷机制Key words
Phosphate-solubilizing strain/Pseudomonas indica/Screening and identification/Saline-alkali tolerance/Organ-ic acid/Phosphate-solubilizing mechanism分类
生物科学引用本文复制引用
李全胜,杨鹏,于航,吕宁,刘瑜,何江勇,樊庆鲁,王国栋..一株解磷印度假单胞菌YWIP10的筛选鉴定及其耐盐碱与解磷特性[J].寒旱农业科学,2026,5(9):848-855,8.基金项目
国家自然科学基金(32460804) （32460804）
国家重点研发项目(2023YFD1900405-02) （2023YFD1900405-02）
院士人才资金项目(2024YS-RC02) （2024YS-RC02）
兵团农业核心技术攻关项目(2023AA601) （2023AA601）
兵团英才支持计划青年项目(2025) （2025）
新疆农垦科学院创新工程项目(NCG202513、NCG202608) （NCG202513、NCG202608）
