果树资源学报2026，Vol.7Issue(5)：9-12,4.
不同大量元素浓度配比对新疆野苹果试管苗生长及扩繁的影响
Effects of Different Macroelement Concentration Ratios on Growth and Propagation of Malus sieversii Plantlets in Vitro
摘要
Abstract
[Objective]The regulatory effects of macronutrient concentration ratios in MS medium on the growth charac-teristics,proliferation efficiency and physiological indices of in vitro plantlets were explored to optimize the in vitro rapid propa-gation system of Malus sieversii.[Methods]Plantlets of Malus sieversii were used as test materials,and a single-factor com-pletely randomized design was adopted.Five macronutrient concentration gradient treatments were set up.Each treatment was supplemented with 0.6 mg/L BA and 0.1 mg/L IBA,and hormone-free MS medium was taken as the control(CK).After 30 days of culture,indicators including proliferation coefficient,plant height,stem diameter,internode length and relative chloro-phyll content(SPAD value)were measured.[Results]Macronutrient concentration ratios exerted significant effects on plant-let growth.Treatment T4 with 1.8-fold macronutrients had the highest proliferation coefficient(6.43),and presented bal-anced performance in plant height(39.6 mm),stem diameter(0.84 mm)and chlorophyll content(40.95 SPAD).Principal component analysis showed that the T4 treatment could synergistically improve proliferation efficiency and physiological indi-ces,and effectively balance plant growth vigor and robustness.[Conclusion]An efficient and low-cost rapid propagation tech-nical system was successfully established,which provides a theoretical basis for the large-scale production of Malus sieversii rootstocks.关键词
新疆野苹果/试管苗/大量元素优化/扩繁系数/主成分分析Key words
Malus sieversii/plantlets in vitro/macroelement optimization/proliferation coefficient/principal component analysis分类
农业科技引用本文复制引用
李媚,马建芳,杨俊霞,王颖..不同大量元素浓度配比对新疆野苹果试管苗生长及扩繁的影响[J].果树资源学报,2026,7(5):9-12,4.基金项目
甘肃省科技厅技术创新引导计划乡村振兴专项(24CXNE005) （24CXNE005）
