甘肃中医药大学学报2026，Vol.43Issue(4)：1-7,7.DOI:10.16841/j.issn1003-8450.2026.04.01
黄芪多糖介导银纳米粒子的制备及其抑菌与抗氧化性能评价
Preparation of Astragalus polysaccharide-mediated silver nanoparticles and evaluation of their antibacterial and antioxidant activities
摘要
Abstract
Objective To develop a green synthesis method for silver nanoparticles(AgNPs)with good disper-sibility and stability using Astragalus polysaccharides(APS),and to evaluate their antioxidant activity,long-term an-tibacterial activity,and blood cell compatibility.Methods AgNPs were synthesized via a one-step reaction of APS(as both reducing and stabilizing agent)with AgNO3 solution at 80 ℃.The resulting nanoparticles were characte-rized using UV-Vis spectroscopy,Fourier transform infrared spectroscopy(FTIR),transmission electron microscopy(TEM),and laser size analysis to examine their structure,morphology,and particle size distribution.Antioxidant ac-tivity was assessed by DPPH and ABTS+radical scavenging assays.Antibacterial activity against Staphylococcus au-reus and Escherichia coli,along with the minimum inhibitory concentration(MIC),was evaluated using the agar well diffusion and gradient concentration methods.Blood cell compatibility was determined via a hemolysis assay.Results The UV-Vis spectrum showed a characteristic surface plasmon resonance peak at 440 nm,confirming the successful formation of AgNPs with an average particle size of about 55.84 nm.The physical adsorption of APS effectively prevented particle aggregation,and no significant increase in particle size was observed after 30 days of storage at room temperature.In the antioxidant assays,AgNPs scavenged DPPH and ABTS+free radicals in a con-centration-dependent manner,with maximum scavenging rates of 89.2％and 96.4％,respectively.Antibacterial tests revealed that APS-mediated AgNPs maintained stable antibacterial activity after 30 days,significantly outperforming those prepared by chemical reduction,with MIC values of 150 μg/mL against S.aureus and 100 μg/mL against E.coli.The hemolysis rate of APS-mediated AgNPs was 3.8％,below the 5.0％safety threshold.Conclusion APS can serve as both a reducing and stabilizing agent for the one-step green synthesis of AgNPs with good dispersibili-ty,stability,antioxidant activity,long-term antibacterial activity,and excellent Hemocytocompatibility,holding strong promise for applications in biomedicine and food preservation.关键词
黄芪多糖/银纳米粒子/长效抑菌活性/抗氧化性能/血细胞相容性Key words
Astragalus polysaccharides/silver nanoparticles/long-term antibacterial activity/antioxidant activi-ty/blood cell compatibility分类
医药卫生引用本文复制引用
杜道全,高小丽,施小宁,李琳,张皓翔,刘文丽,高建德..黄芪多糖介导银纳米粒子的制备及其抑菌与抗氧化性能评价[J].甘肃中医药大学学报,2026,43(4):1-7,7.基金项目
甘肃省教育科技创新项目(2022A-066,2023B-103) （2022A-066,2023B-103）
国家自然科学基金地区项目(82060821). （82060821）
