国际老年医学杂志2026，Vol.47Issue(5)：574-582,9.DOI:10.3969/j.issn.1674-7593.2026.05.004
辣椒素调节TGF-β1/Smad通路对老龄高血压大鼠心肌重构的影响研究
Study on the effect of capsaicin regulating the TGF-β1/Smad pathway on myocardial remodeling in elderly hypertensive rats
摘要
Abstract
Objective To investigate the effect of capsaicin on myocardial remodeling in elderly hypertensive rats and ex-plore its mechanism of action based on the transforming growth factor(TGF)-β1/Smad pathway.Methods Spontaneously hyperten-sive rats and Wistar-Kyoto rats were raised to 16 months of age,and 12 Wistar-Kyoto rats were set as the normal group.Sixty spontane-ously hypertensive rats were randomly divided into the ASH group,Capsaicin-L group,Capsaicin-M group,Capsaicin-H group and Valsartan group,with 12 rats in each group.Capsaicin-L,Capsaicin-M and Capsaicin-H groups were respectively given Capsaicin by gavage at concentrations of 0.05 μmol/kg,0.10 μmol/kg and 0.20 μmol/kg,while the Valsartan group was given valsartan in rats by gavage at concentrations of 30 mg/kg.All groups were administered once a day for 8 consecutive weeks.The systolic blood pressure of the tail artery in rats was measured by a non-invasive sphygmomanometer,and the left ventricular end-diastolic diameter(LVEDD),left ventricular end-systolic diameter(LVESD),ventricular septal end-diastolic thickness(IVSD),left ventricular ejection fraction(LVEF),and left ventricular short-axis systolic rate(LVFS)were measured by ultrasound.The enzyme-linked immunosorbent assay kit was used to determine serum TGF-β1,matrix metalloproteinase(MMP)-9,tumor necrosis factor(TNF)-α,interleukin(IL)-6,and myocardial tissue malondialdehyde(MDA),superoxide dismutase(SOD),angiotensin Ⅱ(Ang Ⅱ),aldosterone(ALD),and atrial natriuretic peptide(ANP),determination of cardiac index and left ventricular index,hematoxylin-eosin(HE)staining to examine myocardial pathological changes,Western blotting to determine the protein levels of transient receptor potential vanilloid type(TRPV)1,TGF-β1,phosphorylated Mothers against decapentaplegic homolog 3(p-Smad3),Smad3,phosphorylated Mothers a-gainst decapentaplegic homolog 7(p-Smad7)and Smad7 in myocardial tissue.Results Capsaicin dose-dependently reduced systolic blood pressure,LVEDD,LVESD,IVSD,serum inflammatory factor levels,MDA,myocardial AngⅡ,ALD,ANP levels,and myo-cardial tissue TGF-β1 and p-Smad3 levels in aged hypertensive rats(P<0.05),while increasing LVEF,LVFS,SOD,myocardial tis-sue TRPV1,and p-Smad7 levels(P<0.05).Conclusion Capsaicin can reduce systolic blood pressure in elderly hypertensive rats,improve cardiac function in rats,alleviate myocardial inflammatory injury and oxidative stress,and inhibit myocardial remodeling.The mechanism may be related to the regulation of the TGF-β1/Smad pathway.关键词
辣椒素/转化生长因子-β1/Smad通路/高血压/心肌重构Key words
Capsaicin/Transforming growth factor-β1/Smad pathway/Hypertension/Myocardial remodeling引用本文复制引用
熊枫,陈乔..辣椒素调节TGF-β1/Smad通路对老龄高血压大鼠心肌重构的影响研究[J].国际老年医学杂志,2026,47(5):574-582,9.基金项目
华中科技大学同济医学院附属梨园医院院内科学研究基金资助项目(2023LYYYHLZX0001) （2023LYYYHLZX0001）
