医学分子生物学杂志2026，Vol.23Issue(5)：485-491,7.DOI:10.3870/j.issn.1672-8009.2026.05.002
Sat1在顺铂诱导HEI-OC1细胞氧化应激中的作用及机制
Role and Mechanism of Sat1 in Cisplatin-Induced Oxidative Stress in HEI-OC1 Cells
摘要
Abstract
Objective To investigate the role and mechanism of spermidine/spermine N1-acetyl-transferase 1(Sat1)in cisplatin(DDP)-induced injury of mouse cochlea-derived immortalized audi-tory epithelial cells(HEI-OC1).Methods HEI-OC1 cells were divided into four groups:siNC,siSat1,siNC+DDP,and siSat1+DDP.Cell proliferation was measured by CCK-8 assay;intracellu-lar reactive oxygen species(ROS)levels were detected using DCFH-DA fluorescent probe;mRNA expression levels of Sat1,catalase(CAT),and glutathione peroxidase 1(GPx1)were assessed by qPCR;protein expression of Sat1 and apoptosis-related proteins,including B-cell lymphoma-2(Bcl-2),Bcl2-associated X protein(Bax),cysteinyl aspartate-specific proteinase-3(Caspase-3),and cleaved Caspase-3,were detected by Western blotting,and the ratios of cleaved Caspase-3/Caspase-3 and Bcl-2/Bax were calculated.Malondialdehyde(MDA)content and superoxide dismutase(SOD)activity were measured by ELISA to evaluate oxidative stress.Results Compared with the siNC group,the siSat1 group showed downregulated Sat1 mRNA and protein expression,decreased basal ROS and MDA levels,increased SOD activity and CAT and GPx1 mRNA expression,and reduced Bax and Cleaved Caspase-3 protein expression(all P<0.05).After DDP treatment,the siNC+DDP group exhibited decreased cell viability,elevated ROS and MDA levels,reduced SOD activity and CAT and GPx1 mRNA expression,and increased Bax and cleaved Caspase-3 protein expression;all these alterations were significantly reversed in the siSat1+DDP group(all P<0.05).Conclusion Sat1 plays a critical role in DDP-induced HEI-OC1 cell apoptosis by mediating oxida-tive stress.关键词
精胺/亚精胺N1-乙酰转移酶1/顺铂/氧化应激/细胞凋亡Key words
spermidine/spermine N1-acetyltransferase 1/cisplatin/oxidative stress/apoptosis分类
医药卫生引用本文复制引用
蒋慧,侯小娟,刘静,丁伟,吴梅..Sat1在顺铂诱导HEI-OC1细胞氧化应激中的作用及机制[J].医学分子生物学杂志,2026,23(5):485-491,7.基金项目
新疆维吾尔自治区人民医院院内项目(No.20240201),"天山英才"医药卫生高层次人才培养计划(No.TSYC202301B030),新疆第二医学院2025年校级科研基金项目(No.ZR202553) This work was supported by grants from the Hospital Research Project of People's Hospital of Xinjiang Uygur Autonomous Region(No.20240201),the"Tianshan Talents"Training Program for High-Level Medical and Health Talents(No.TSYC202301B030),and the 2025 University-Level Scien-tific Research Fund of Xinjiang Second Medical College(No.ZR202553). （No.20240201）
