医学分子生物学杂志2026，Vol.23Issue(5)：502-513,12.
结直肠癌不同原发部位肠道微生物组的宏基因组学荟萃分析
Metagenomic Meta-Analysis of Gut Microbiome in Different Primary Locations of Colorectal Cancer
摘要
Abstract
Objective The intestinal microbiota is related to the primary site of colorectal can-cer(CRC).To explore the differences in the intestinal microbiome among different primary sites of colorectal cancer,this study conducted a meta-analysis using comprehensive genomic data.Meth-ods Genomic data were collected from three studies and grouped according to three sites:right-sided colon cancer(RCC),left-sided colon cancer(LCC),and rectal cancer(RC).Firstly,con-founding factors were identified through two-factor variance analysis and comparison of microbial diversity.Subsequently,a microbial co-occurrence network was constructed.A random forest algo-rithm was used to build a prediction model for the location of the primary tumor.Finally,tumor loca-tion and confounding factors were incorporated into the Maaslin2(microbiome multivariable associa-tions with linear models)model and linear discriminant analysis(LDA)was used to identify differ-entially expressed species.Results The sources of research and BMI are the main confounding fac-tors.There is no significant difference in the α/β diversity of intestinal microbiota among different tumor sites.The Actinobacteria phylum,Firmicutes phylum,and Proteobacteria phylum all play key linking roles in the three microbial networks,and the Bacteroidetes phylum is more significant in the microbial network of RCC.There are both common key bacterial groups and their own unique key bacterial groups in the three networks.The random forest classification model performs well in predicting RC,but performs poorly in distinguishing RCC from LCC.By comparing CC with RC,and comparing RCC with LCC,some of the differential bacterial groups identified by Maaslin2 and LDA have been reported to be related to molecular and immune characteristics of CRC location.Conclusion This study elucidated the relationship between gut microbiome and CRC location and confirmed that RCC,LCC,and RC had different enrich patterns of microbiota.关键词
宏基因组学/微生物组/结直肠癌/肿瘤位置/荟萃分析Key words
metagenomic/microbiome/colorectal cancer/tumor location/meta-analysis分类
医药卫生引用本文复制引用
廖之诚,龙夏薇,黎倩,王宗佳,林有智,李永强,廖小莉..结直肠癌不同原发部位肠道微生物组的宏基因组学荟萃分析[J].医学分子生物学杂志,2026,23(5):502-513,12.基金项目
广西重点研发计划项目(No.桂科AB25069073),广西自然科学基金(No.2023GXNSFAA026156、No.2023GXNSFAA026145),国家自然科学基金地区基金(No.82060545) This work was supported by grants from Guangxi Key Research and Development Program(No.Gui Ke AB25069073),Natural Science Foundation of Guangxi Province(No.2023GXNSFAA026156,No.2023GXNSFAA026145),Regional Fund of National Natural Science Foundation of China(No.82060545). （No.桂科AB25069073）
