医学分子生物学杂志2026，Vol.23Issue(5)：531-540,10.DOI:10.3870/j.issn.1672-8009.2026.05.007
白藜芦醇通过SRC/FGFR1轴改善糖尿病足的分子机制研究
Resveratrol Ameliorates Diabetic Foot via the SRC/FGFR1 Axis
摘要
Abstract
Objective To investigate the molecular mechanism through which resveratrol(RES)modulates the progression of diabetic foot by targeting SRC proto-oncogene,non-receptor tyrosine kinase(SRC)/fibroblast growth factor receptor 1(FGFR1)axis.Methods Human dermal fibroblasts(HDF)were allocated into NG(normal glucose),HG(high glucose),and HG+RES groups.Cell proliferative viability was measured by CCK-8 assay,migration by Transwell assay,and apoptosis by TUNEL staining.Bioinformatic analyses were performed to predict potential targets of RES in diabetic foot and to explore the associated signaling pathways.HDF cells were further divided into NG,HG,HG+si-SRC,HG+oe-SRC,and HG+oe-SRC+si-FGFR1 groups to determine how alterations in SRC and FGFR1 expression affect proliferative viability,migratory capacity,and apoptosis.A diabetic foot animal model was established and treated with RES;α-lipoic acid served as the positive control to evaluate the therapeutic potential of RES.Results Bioinformatic screen-ing identified SRC as one of the intersecting targets of RES and diabetic foot,and as a key molecule in endocrine resistance pathway.Compared with the NG group,HG-treated HDF cells exhibited up-regulated SRC expression,reduced proliferative viability and migratory capacity,and increased apoptosis;these effects were reversed by RES intervention(P<0.05).Relative to the HG group,SRC knockdown enhanced cell viability and migration while decreasing apoptosis,whereas SRC overexpression further suppressed proliferative viability and migration and promoted apoptosis;simultaneous FGFR1 knockdown partially reversed these latter effects(P<0.05).Animal experi-ments confirmed that both RES and α-lipoic acid exert therapeutic efficacy in the diabetic foot model.Conclusion Resveratrol promotes the improvement of diabetic foot by inhibiting the SRC/FGFR1 axis.关键词
白藜芦醇/非受体酪氨酸激酶肉瘤病毒蛋白/成纤维细胞生长因子受体1/糖尿病足Key words
resveratrol/SRC proto-oncogene,non-receptor tyrosine kinase/fibroblast growth factor receptor 1/diabetic foot分类
医药卫生引用本文复制引用
谷雯雯,谢娟..白藜芦醇通过SRC/FGFR1轴改善糖尿病足的分子机制研究[J].医学分子生物学杂志,2026,23(5):531-540,10.基金项目
安徽省卫生健康科研项目(No.AHWJ2023A20229). This work was supported by a grant from the Anhui Provincial Health Research Project(No.AHWJ2023A20229). （No.AHWJ2023A20229）
