广西植物2026，Vol.46Issue(8)：1267-1282,16.DOI:10.11931/guihaia.gxzw202505020
茶树DIR基因家族鉴定及在炭疽病胁迫下表达模式分析
Identification and expression pattern analysis of DIR gene family in Camellia sinensis under anthracnose stress
摘要
Abstract
Dirigent(DIR)proteins play crucial roles in lignin and lignan biosynthesis process,significantly influencing plant growth,development and stress responses.This study identified 40 CsDIR genes through genome-wide screening in Camellia sinensis,and analysed for physicochemical properties,phylogenetic tree,conserved motifs,chromosomal localization,cis-acting element structural features,and gene expression patterns of DIR gene family under tea anthracnose stress.The results were as follows:(1)Phylogenetic analysis showed that the CsDIR genes were divided into three subfamilies,and their promoter regions contained response elements for auxin,abscisic acid,wounding and pathogens.(2)Analysis of protein structures and protein conserved domains confirmed that 40 CsDIRs contained a β-barrel composed of eight antiparallel β-strands.In addition,39 CsDIRs possessed a Dirigent domain,while one contained a Dirigent superfamily domain.(3)Results of chromosomal localization and collinearity analysis indicated that 40 CsDIR genes were distributed across 10 chromosomes and 5 distinct Contigs.Within CsDIR gene family,we identified 12 pairs of tandemly duplicated genes and 4 pairs of collinear genes.(4)Expression pattern analysis of 12 CsDIR genes under tea anthracnose stress demonstrated that all 12 CsDIR genes uniformly responded to tea anthracnose stress.This study provides a foundation for future research on the function of DIR gene family in response to biotic stresses in tea plants.关键词
茶树/DIR 基因家族/结构特征/理化特性/表达分析Key words
Camellia sinensis/DIR gene family/structural features/physicochemical property/expression analysis分类
农业科技引用本文复制引用
孙志鹏,赵懿琛..茶树DIR基因家族鉴定及在炭疽病胁迫下表达模式分析[J].广西植物,2026,46(8):1267-1282,16.基金项目
国家自然科学基金项目(32160077) （32160077）
贵州省科技计划项目(黔农科院人才专项[2024]02). （黔农科院人才专项[2024]02）
