广西植物2026，Vol.46Issue(8)：1385-1393,9.DOI:10.11931/guihaia.gxzw202511014
黄花棘豆中1个新的黄酮苷化合物及生物活性研究
A new flavonoid glycoside from Oxytropis ochrocephala and its biological activities
摘要
Abstract
To investigate the chemical constituents of Oxytropis ochrocephala and their antioxidant activities,the ethanol extract of O.ochrocephala was separated and purified using chromatographic techniques including silica gel column chromatography,Sephadex LH-20 chromatography and semi-preparative high-performance liquid chromatography.The structures of the isolated compounds were elucidated by comprehensive spectroscopic analyses,including UV,IR,HR-MS and NMR.The antioxidant activities of the isolated compounds were evaluated using 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2-azinobis-(3-ethyl-benzothiazole-6-sulfonic acid)(ABTS)radical scavenging assays.The results were as follows:(1)Twelve compounds were isolated and identified as ochrocephalamine Q(1),(6aR,11aR)-10-hydroxy-3,9-dimethoxypterocarpan(2),3',7-dihydroxy-2',4'-dimethoxyisoflavan(3),bosenegaloside A(4),5-hydroxy-2-(4-hydroxyphenyl)-7-methoxy-3-[6-O-(1-oxohexadecyl)-α-D-altropyranosyl]-4H-1-benzopyran-4-one(5),rhamnocitrin(6),3,5,7-trihydroxy-4'-methoxyflavonol(7),3-O-rhamnocitrin-6-O-benzoyl-β-D-glucopyranoside(8),rhamnocitrin-3-O-β-D-galactoside(9),isoquercitrin(10),rhamnetin 3-O-β-D-galactoside(11)and rhamnocitrin 3-O-β-D-glucoside(12).Among these,Compound 1 was a new compound,while compounds 2,4 and 5 were reported in this species for the first time.(2)Compounds 6,10 and 11 exhibited notable antioxidant activities in the DPPH radical scavenging assay,with IC50 values of(4.90±0.35),(0.80±0.06)and(1.07±0.02)μg·mL-1,respectively,which were stronger than that of the positive control vitamin C[IC50 value of(5.72±0.52)μg·mL-1].In addition,in the ABTS radical scavenging assay,Compound 11 also showed strong antioxidant activity,with an IC50 value of(0.32±0.05)μg·mL-1.These findings further enrich the chemical constituents of O.ochrocephala and provide valuable information for its potential resource development and utilization.关键词
黄花棘豆/黄酮/化学成分/自由基清除/抗氧化Key words
Oxytropis ochrocephala/flavonoids/chemical constituents/free radical scavenging/antioxidant分类
农业科技引用本文复制引用
杨敏,谭晶,杨孙缘,熊荣琴,曾艳荣,张振,路浩,谭承建..黄花棘豆中1个新的黄酮苷化合物及生物活性研究[J].广西植物,2026,46(8):1385-1393,9.基金项目
国家自然科学基金项目(32160110,31660103) （32160110,31660103）
贵州省研究生教育创新计划项目(2024YJSKYJJ225). （2024YJSKYJJ225）
