高原农业2026,Vol.10Issue(4):461-467,7.DOI:10.19707/j.cnki.jpa.2026.04.004
黄蝉兰与长叶兰杂交试验及其种子无菌萌发
Hybridization Experiment of Cymbidium iridioides and Cymbidium erythraeum and Hybrid Seed Aseptic Germination
摘要
Abstract
In order to get a new cultivar of Cymbidium,a hybridization experiment was conducted between Cymbidium iridioides and C.erythraeum,and hybrid seeds from C.iridioides×C.erythraeum were cultured under aseptic conditions.The results showed:1.capsules can form up-to 100%through cross or reciprocal cross between C.iridioides and C.erythraeum,but maturation periods differ,which shows dominance of maternal genetic inheritance,and hyper-parental inheritance phenomenon with delayed maturity.2.The embryo rates of seeds from C.iridioides×C.erythraeum were 99.16%(cross)and 100%(reciprocal cross);respectively,with low variation coefficients and stable seed morphology.3.Under experimental conditions,four culture media-1/2 MS,MS,KC,and Hyponex NO.1-supplemented with 30 g/L sucrose,7 g/L agar,and 1 g/L activated carbon,all enabled the aseptic germination to protocorms of hybrid seeds from C.iridioides×C.erythraeum.However,KC was proved to be the most suitable culture medium,with a 35.17%germination rate.关键词
黄蝉兰/长叶兰/杂交育种/种子形态/无菌萌发Key words
Cymbidium iridioides/Cymbidium erythraeum/Cross-breeding/Seed morphology/Aseptic germination分类
农业科技引用本文复制引用
赵珺雯,王瑞,朱荣杰,王芳,邢震..黄蝉兰与长叶兰杂交试验及其种子无菌萌发[J].高原农业,2026,10(4):461-467,7.基金项目
西藏自治区科技计划项目:雅江下游濒危兰属资源抢救性保护与人工繁育体系构建(XZ202401ZY0036). (XZ202401ZY0036)