海南医科大学学报2026，Vol.32Issue(17)：1281-1289,9.DOI:10.13210/j.cnki.jhmu.20250624.002
TrxA基因对类鼻疽伯克霍尔德菌生物学活性的影响
Effects of the TrxA gene on the biological activities of Burkholderia pseudomallei
摘要
Abstract
Objective:To investigate the biological function of TrxA in Burkholderia pseudomallei(B.pseudomallei),with a focus on exploring the role of TrxA in regulating B.pseudomallei's biological activities.Methods:A TrxA gene deletion mutant(ΔTrxA)was constructed using homologous recombination,and a complemented strain(ΔTrxA∷TrxA)was obtained via elec-troporation.Using the wild-type(WT)B.pseudomallei strain as a control,systematically evaluated differences among the three strains in terms of growth,motility,biofilm formation,antibiotic resistance,oxidative stress tolerance,and infectivity.Specific as-says included:growth curve analysis,point plate and area measurement methods were used to evaluate motility,crystal violet staining was used to detect biofilm formation,drug sensitivity plate was used to test antibiotic susceptibility,the Kirby-Bauer method was used to evaluate oxidative stress tolerance,and colony counting and CCK-8 assays were used to assess bacterial adhe-sion and cytotoxicity to the host cells.Results:Compared to the WT and ΔTrxA∷TrxA strains,the ΔTrxA mutant exhibited sig-nificantly reduced growth and motility(P<0.05),as well as significantly decreased infectivity and cytotoxicity toward the host cells(P<0.05).Regarding biofilm formation,the ΔTrxA strain formed a more robust biofilm than the WT strain at 24 hour.However,by the 72 hour,no statistical significant differences were observed among the three strains(P>0.05).The ΔTrxA strain showed increased resistance to certain antibiotics,while there were no statistical significant differences in tolerance to oxidative stress in-duced by hydrogen peroxide among all three strains(P>0.05).These findings suggested that TrxA plays a critical role in B.pseu-domallei's biological activity,as well as infectivity and cytotoxic against the host cells.Conclusion:TrxA plays a key role in regu-lating the growth,motility,biofilm formation,infectivity,and cytotoxicity of B.pseudomallei.This study provides a theoretical basis for further elucidating the pathogenic mechanisms of B.pseudomallei and developing novel therapeutic targets.关键词
类鼻疽伯克霍尔德菌/生物活性/感染/TrxA蛋白Key words
Burkholderia pseudomallei/Biological activity/Infection/TrxA protein分类
医药卫生引用本文复制引用
李欣泽,刘双媛,宋兆杰,夏乾峰,李雪霞..TrxA基因对类鼻疽伯克霍尔德菌生物学活性的影响[J].海南医科大学学报,2026,32(17):1281-1289,9.基金项目
This study was supported by the National Natural Science Foundation of China(82060001) （82060001）
Hainan Medical University Student Innovation and Entrepreneurship Project(S202411810006) 国家自然科学基金(82060001) （S202411810006）
海南医科大学大学生创新创业项目(S202411810006) （S202411810006）
