黑龙江动物繁殖2026，Vol.34Issue(4)：1-8,8.DOI:10.19848/j.cnki.ISSN1005-2739.2026.08.0001
母牛发情前后阴道菌群组成及潜在功能变化分析
Changes in vaginal microbiota composition and potential functions from estrus to post-estrus in cows
摘要
Abstract
To investigate the dynamic changes and potential functional characteristics of the vaginal microbiota during estrus in cows,ten healthy Simmental cows with similar body condition were selected as the study subjects.A paired longitudinal sampling design was adopted,in which vaginal swab samples were collected from the same cows during estrus(E group)and 48 h after estrus(PE group).The diversity,community structure,and composition of the vaginal microbiota at the two physiological stages were analyzed using 16S rRNA gene sequencing.Differential genera were analyzed using STAMP and LEfSe,and the potential functions of the vaginal microbiota were predicted using PICRUSt.The results showed that 10 759 and 9 132 OTUs were obtained in the E and PE groups,respectively,with 3 635 OTUs shared between the two groups.PCoA showed substantial overlap between samples from the two groups in the ordination space.No significant differences were observed in the Ace,Chao1,Shannon,or Simpson indices(P>0.05),indicating that the overall richness,diversity,and community structure of the vaginal microbiota did not change markedly between the two stages.At the phylum level,Proteobacteria,Bacteroidetes,and Firmicutes were the dominant phyla in both groups.At the genus level,the relative abundances of Lactobacillus,Escherichia-Shigella,and Parabacteroides were significantly lower in the PE group(P<0.05),whereas those of BD1-7 clade,Coprococcus 2,and Terrisporobacter were significantly higher(P<0.05).LEfSe analysis further identified differential microbial taxa characteristic of the E and PE stages.PICRUSt functional prediction revealed differences between the two stages in potential functional categories including carbohydrate metabolism,amino acid metabolism,and cofactor and vitamin metabolism.关键词
西门塔尔母牛/发情期/发情后期/阴道菌群/16S rRNAKey words
Simmental cows/estrus/post-estrus/vaginal microbiota/16S rRNA分类
农业科技引用本文复制引用
徐静怡,何贵天,王洁茹,方翟,高庆华..母牛发情前后阴道菌群组成及潜在功能变化分析[J].黑龙江动物繁殖,2026,34(4):1-8,8.基金项目
新疆生产建设兵团科技计划项目(2026DB041) （2026DB041）
塔里木大学校长基金项目(TDZKBS202628) （TDZKBS202628）
