黑龙江动物繁殖2026，Vol.34Issue(4)：9-14,6.DOI:10.19848/j.cnki.ISSN1005-2739.2026.08.0002
E2、P4与维生素ADE预处理及受体不同CIDR撤栓时序对西门塔尔牛超数排卵和同期发情的影响
Effects of donor pretreatment with E2,P4 and vitamin ADE and different CIDR removal timings in recipients on superovulation and estrus synchronization in Simmental cattle
摘要
Abstract
This study aimed to investigate the effects of estradiol(E2),progesterone(P4),and vitamin ADE pretreatment in donor cows on superovulatory responses in Simmental cattle,as well as the effects of different CIDR removal timings in recipient cows on estrus synchronization between donors and recipients,with the objective of providing a reference for optimizing superovulation and embryo transfer protocols in Simmental cattle.Two experimental protocols were conducted.In protocol Ⅰ,24 healthy Simmental donor cows were randomly assigned to two groups under identical feeding and management conditions.Group 1(n=14)received an intramuscular injection of 2 mg E2+100 mg P4+10 mL ADE at the time of CIDR insertion,whereas group 2(n=10)received CIDR insertion only.Thereafter,both groups underwent the same decreasing-dose FSH superovulation protocol.The numbers of mature follicles,ovulations,and viable embryos were compared between the two groups.In Protocol Ⅱ,10 Simmental cows were selected as donors and 50 as recipients.The recipient cows were randomly divided into three groups according to CIDR removal timing relative to the donor cows:24 h earlier,12 h earlier,or simultaneously(0 h).The onset and end of estrus were recorded for each group,and the differences in estrus timing between donors and recipients were compared.The results showed that,in protocol Ⅰ,the numbers of mature follicles and ovulations in group 1 were 16.93±3.81 and 11.29±3.50,respectively,both of which were significantly higher than those in group 2,respectively(12.90±2.77 and 7.90±2.73,P<0.05).Although the number of viable embryos in group 1(8.64±2.65)was higher than that in group 2(6.40±2.67),the difference did not reach statistical significance(P=0.054).In protocol Ⅱ,group C(CIDR was removed 12 h earlier in recipients than in donors)showed a 1 h difference with the donor group,whereas group B(24 h earlier group)and group D(simultaneous-removal group)showed differences of 4 h and 5 h,respectively.These results indicate that pretreatment of donor cows with E2+P4+vitamin ADE at the initial stage of CIDR insertion can significantly increase the numbers of mature follicles and ovulations.In recipient cows,PG administration combined with CIDR removal 12 h earlier than in donor cows can achieve a high degree of estrus synchronization between donors and recipients.关键词
西门塔尔牛/超数排卵/胚胎移植/雌二醇/黄体酮/撤栓时间Key words
Simmental cattle/superovulation/embryo transfer/estradiol/progesterone/CIDR removal timing分类
农业科技引用本文复制引用
张磊,刘佳楠,牛鹏,李小鹏,王洁茹,黄飞,方翟,韩春梅..E2、P4与维生素ADE预处理及受体不同CIDR撤栓时序对西门塔尔牛超数排卵和同期发情的影响[J].黑龙江动物繁殖,2026,34(4):9-14,6.基金项目
新疆生产建设兵团科技计划项目-重点领域科技攻关计划项目项目(2025AB082) （2025AB082）
