黑龙江动物繁殖2026，Vol.34Issue(4)：28-33,6.DOI:10.19848/j.cnki.ISSN1005-2739.2026.08.0006
牛OPU-IVEP效率影响因素分析
Analysis of factors affecting the efficiency of bovine OPU-IVEP
摘要
Abstract
The aim of this experiment was to analyze the effects of in vitro culture methods,pituitary FSH(pFSH)pre-treatment,and physiological status on Holstein bovine OPU-IVEP efficiency.In the experiment,two different in vitro culture systems(single-step and two-step culture methods),pFSH from different sources of(N-pFSH,S-pFSH,and B-pFSH),and different physiological conditions(non-pregnant and early pregnant)were chosen to evaluate the developmental competence of OPU-derived oocytes in Holstein bovine.The results showed that compared with the single-step culture method,the two-step culture method could significantly increase the cleavage rate[(82.7±3.8)%vs.(75.6±5.7)%,P<0.05]and A-grade blastocyst rate[(37.2±2.7)%vs.(27.8±2.9)%,P<0.05],and no significant difference in the blastocyst rate between the two culture methods[(55.1±2.6)%vs.(49.2±1.8)%,P>0.05].Compared with S-pFSH and B-pFSH,N-pFSH pre-treatment significantly increased the average number of viable oocytes[(26.5±2.9)%vs.(17.3±2.2)%vs.(15.8±2.6)%,P<0.05],and blastocyst rates and A-grade blastocyst rate were similar among all groups(P>0.05).In addition,the similar average number of viable oocytes,cleavage rate,blastocyst rate and A-grade blastocyst rate in early-pregnancy donor was observed,compared to non-pregnancy donor(P>0.05).In summary,N-pFSH pre-treatment combined with the two-step culture method could improve the OPU-IVEP efficiency,and early-pregnancy cows can also be effectively used for OPU-IVEP.关键词
荷斯坦牛/活体采卵-体外胚胎生产/体外培养方式/垂体促卵泡素/妊娠Key words
Holstein bovine/OPU-IVEP/in vitro culture method/pituitary FSH/pregnancy分类
农业科技引用本文复制引用
宋宇琨,郜欣欣,康银,范翠婷,冯春涛,张家新..牛OPU-IVEP效率影响因素分析[J].黑龙江动物繁殖,2026,34(4):28-33,6.基金项目
内蒙古自治区揭榜挂帅项目(2022JBGS0025) （2022JBGS0025）
