黑龙江动物繁殖2026，Vol.34Issue(4)：34-44,11.DOI:10.19848/j.cnki.ISSN1005-2739.2026.08.0007
猪体细胞核移植中使用显示器可视化操作的可行性
The feasibility of using display devices to visualize operations in porcine somatic cell nuclear transfer
摘要
Abstract
To explore the work-load characteristics of personnel engaged in long-term micromanipulation experiments and evaluate the effects of display-visualized micromanipulation on operating posture,operational efficiency and the identification of early embryonic developmental indicators in porcine somatic cell nuclear transfer(SCNT),questionnaire surveys and ergonomic assessment methods were conducted in this study for a comparison of work-related discomforts among micromanipulation operators,working posture,operation duration and the identification of early development of SCNT reconstructed embryos between the traditional eyepiece-based operation and the display-visualized operation.The data of the operational workload of 62 technicians performing porcine embryo micromanipulation was collected,and the developmental outcomes of porcine SCNT reconstructed embryos under traditional eyepiece micromanipulation and display-visualized micromanipulation were used for comparison.The results showed that technicians performing long-term traditional eyepiece micromanipulation mainly suffered from musculoskeletal discomfort,visual fatigue and mental fatigue,in which shoulder-neck soreness,low-back pain,lack of concentration and eye strain were listed in the most prevalent complaints,and significant positive correlations were observed among different fatigue symptoms(P<0.05).Compared with traditional eyepiece micromanipulation,display-visualized operation significantly reduced neck flexion angle(from 41.0°±1.0° to 6.24°±0.94°)and trunk forward inclination angle(from 20.0°±3.0° to 10.16°±1.10°),showing improved working posture during embryo manipulation.However,the time consumed for relevant micromanipulation steps increased significantly under display-visualized operation.Meanwhile,no significant differences were found in the cleavage rate and blastocyst rate of porcine SCNT reconstructed embryos derived from the two operation approaches(P>0.05).In conclusion,the display-visualized micromanipulation method is applicable to porcine SCNT embryo production,could effectively improve operating conditions without compromising the early developmental competence of reconstructed embryos,and may bring about prolonged operation time under current experimental conditions.关键词
猪/体细胞核移植/胚胎生产/显示器可视化操作/重构胚/胚胎发育Key words
porcine/somatic cell nuclear transfer/embryo production/monitor-aided visual manipulation/reconstructed embryo/embryonic development分类
生物科学引用本文复制引用
Umar Saeed,王锋冲,陈曦,郭建雄,奎华,魏红江..猪体细胞核移植中使用显示器可视化操作的可行性[J].黑龙江动物繁殖,2026,34(4):34-44,11.基金项目
科技创新2030"农业生物育种"重大项目(2023ZD0407503) （2023ZD0407503）
