河南农业科学2026，Vol.55Issue(9)：103-115,13.DOI:10.15933/j.cnki.1004-3268.2026.09.010
油莎豆乙酰辅酶A羧化酶基因家族的鉴定及CeACCase2功能解析
Genome-Wide Identification of Acetyl-CoA Carboxylase Gene Family in Cyperus esculentus and Functional Characterization of CeACCase2
摘要
Abstract
Cyperus esculentus L.is an emerging oil crop and one of the few known crops capable of synthesizing large amount of oil in underground tubers.Elucidating the genetic mechanisms underlying tuber oil biosynthesis is of great significance for molecular breeding of C.esculentus.Acetyl-CoA carboxylase(ACCase),a key rate-limiting enzyme catalyzing the first committed step of fatty acid biosynthesis,plays an important role in oil synthesis.In this study,using the cultivar Yuyousha No.2 as the experimental material,members of C.esculentus ACCase gene family(CeACCases)were identified based on genomic data.Their physicochemical properties,subcellular localization,phylogenetic relationships,conserved motifs,and promoter cis-acting elements were systematically analyzed using bioinformatics methods.The expression patterns of each member in different tissues were examined by q RT-PCR.The key gene CeACCase2 was cloned,and its subcellular localization and heterologous overexpression functional verification in Arabidopsis thaliana were performed.The results showed that a total of 13 CeACCases members were identified from C.esculentus.Their encoded proteins were mainly localized in chloroplasts,with significant differences in physicochemical properties.Phylogenetic and conserved motif analyses divided the family members into five subfamilies,among which the homomeric members possessed the most complete conserved motifs.The promoter regions were enriched with oil synthesis-related cis-acting elements.qRT-PCR analysis revealed that CeACCases members exhibited relatively high expression levels in tubers.Subcellular localization results indicated that the CeACCase2 protein was mainly localized in chloroplasts.Overexpression of CeACCase2 in Arabidopsis significantly increased seed oil content by 13.1 percentage ponits compared with the wild type.In conclusion,this study confirms that CeACCase2 plays a key role in oil biosynthesis,providing theoretical support for elucidating the genetic basis of the tuber oil biosynthesis pathway in C.esculentus.关键词
油莎豆/油脂合成/乙酰辅酶A羧化酶/亚细胞定位/功能验证Key words
Cyperus esculentus/Oil synthesis/Acetyl-CoA carboxylase(ACCase)/Subcellular localization/Functional verification分类
农业科技引用本文复制引用
李春鑫,宋万献,王会伟,王猛,张夏冰,朱雅婧,刘馨逸,翟允汝,陈晨,关超男,王树峰..油莎豆乙酰辅酶A羧化酶基因家族的鉴定及CeACCase2功能解析[J].河南农业科学,2026,55(9):103-115,13.基金项目
河南省农业科学院自主创新项目(2026ZC21,2026ZC17) （2026ZC21,2026ZC17）
河南省农业科学院基础性科研项目(2026JC09) （2026JC09）
