核农学报2026，Vol.40Issue(10)：1918-1928,11.DOI:10.11869/j.issn.1000-8551.2026.10.1918
黑麦NPC基因家族鉴定及苗期胁迫表达分析
Identification of NPC Gene Family and Expression Analysis of Seedling Stress in Rye(Secale cereale L.)
摘要
Abstract
Non-specific phospholipase C(NPC)is an important class of enzymes involved in plant phospholipid metabolism.It catalyzes the hydrolysis of phospholipids to generate diacylglycerol and phosphorylated head groups,thereby playing a crucial role in lipid signal transduction,membrane remodeling,and responses to abiotic stresses during plant growth and development.ScNPCs represent a group of genes family encoding NPC proteins in the genome of rye(Secale cereale L.).Comprehensive bioinformatics analyses were performed to evaluate their physicochemical properties,phylogenetic relationships,conserved motifs and domain structures,as well as cis-acting elements in promoter regions.In addition,their expression patterns were analyzed across different tissues,developmental stages,and under drought and low-temperature stress conditions.Results showed that six ScNPCs genes(ScNPC01-ScNPC06)are distributed across five rye chromosomes,encoding proteins ranging from 509-541 amino acids in length.Their promoter regions are enriched with cis-acting elements related to phytohormone signaling,stress responses,and developmental regulation.Transcriptomic analysis revealed that ScNPCs expressions exhibit pronounced spatiotemporal specificity,with all members being upregulated during post-pollination grain filling stage.Quantitative real-time PCR(qRT-PCR)further revealed that drought stress significantly induces the expression of ScNPC01-ScNPC06,whereas low-temperature stress selectively upregulates ScNPC04 at the middle phase of the treatment,while ScNPC02 and ScNPC03 show a transient rise followed by a subsequent decline.This study provides valuable candidate genes and a theoretical foundation for further functional studies on the NPC gene family in Secale cereale L.in response to diverse stress conditions.关键词
黑麦/NPC家族/全基因组鉴定/非生物胁迫/表达分析Key words
Secale cereale L./NPC family/genome-wide identification/abiotic stress/expression analysis分类
农业科技引用本文复制引用
王晓东,豆飞飞,刘春颖,王石磊,刘彩霞,刘凤楼,王掌军,李清峰..黑麦NPC基因家族鉴定及苗期胁迫表达分析[J].核农学报,2026,40(10):1918-1928,11.基金项目
国家自然科学基金项目(32260467),宁夏回族自治区自然科学基金优秀青年项目(2024AAC05022),农业农村部-农业种质资源普查收集、保护鉴定服务(19240579),宁夏科技重大专项(2022BSB03109) （32260467）
