核农学报2026，Vol.40Issue(10)：1929-1935,7.DOI:10.11869/j.issn.1000-8551.2026.10.1929
小豆锌指转录因子VaPLATZ7在抗病中的功能分析
Functional Analysis of Adzuki Bean Zinc-Finger Transcription Factor VaPLATZ7 in Disease Resistance
摘要
Abstract
To identify potential rust resistance genes in adzuki bean,the expression profiles of VaPLATZ7 were comparatively analyzed between the resistant cultivar LJ01 and the susceptible cultivar BQH after inoculation with Uromyces vignae using quantitative real-time PCR(qRT-PCR).Compared with the uninoculated control,the expression level of VaPLATZ7 in LJ01 increased to 56.9-fold at 12 h post-inoculation(hpi)and remained significantly higher than the control across 24 to 48 h,whereas its expression in BQH was markedly suppressed throughout the same period.Cis-acting element analysis of the VaPLATZ7 promoter identified five methyl jasmonate(MeJA)-responsive motifs.Upon exogenous MeJA treatment,the gene was significantly induced within 2 h,peaking at 4.5-fold relative to the control.Moreover,transient over-expression of VaPLATZ7 in tobacco significantly enhanced plant resistance to Botrytis cinerea infection,resulting in a 15％reduction in lesion diameter compared with the control.Collectively,VaPLATZ7 positively regulates plant disease resistance which is likely modulated via the JA signaling pathway.These findings provide a candidate gene resource that can be further exploited for disease resistance breeding in adzuki bean.关键词
小豆/小豆锈病/抗病基因/转录因子Key words
adzuki bean/adzuki bean rust/resistance gene/transcription factor分类
农业科技引用本文复制引用
张书华,丁欣,孙伟娜,徐晓丹,殷丽华,柯希望..小豆锌指转录因子VaPLATZ7在抗病中的功能分析[J].核农学报,2026,40(10):1929-1935,7.基金项目
国家自然科学基金(32502459),黑龙江省自然科学基金(LH2024C081),黑龙江八一农垦大学研究生创新科研项目(YJSCX2023-ZX07) （32502459）
