核农学报2026，Vol.40Issue(10)：1936-1947,12.DOI:10.11869/j.issn.1000-8551.2026.10.1936
马铃薯StKFB29基因克隆、亚细胞定位及其互作蛋白筛选与分析
Gene Cloning,Subcellular Localization,Screening and Analysis of Interacting Proteins of StKFB29 in Potato
摘要
Abstract
Anthocyanins play a significant role in enhancing stress resistance and nutritional quality of potato.To investigate the anthocyanin metabolic mechanism in potato,we identified a potential regulatory gene,StKFB29,involved in anthocyanin metabolism through transcriptome sequencing of pigmented potato varieties in preliminary studies.To elucidate its function,the StKFB29 gene was successfully cloned in this study,and the subcellular localization of its encoded protein as well as its interacting proteins were analyzed.The results showed that the complete coding sequence(CDS)of StKFB29 is 1 137 bp in length,encoding 378 amino acids,and the protein contains one F-box domain and three Kelch motifs.Subcellular localization experiments confirmed that StKFB29 is localized in the nucleus.Using the yeast two-hybrid system,22 potential interacting proteins of StKFB29 were screened,and the expression patterns of genes encoding these interacting proteins were analyzed across different potato tissues.Furthermore,yeast two-hybrid and bimolecular fluorescence complementation assays confirmed the interactions between StKFB29 and phenylalanine ammonia-lyase(StPAL)as well as UDP-glucuronate decarboxylase 1(StUXS1).These findings provide a theoretical foundation for further deciphering the functions and mechanisms of StKFB29 in plant metabolism and offer important genetic resources for molecular breeding in potato.关键词
马铃薯/StKFB/次生代谢/基因克隆/蛋白互作Key words
potato/StKFB/secondary metabolism/gene cloning/protein interaction分类
农业科技引用本文复制引用
单虔文,王轶楠,吴美玲,兰小美,贺立恒,贾小云,白小东,唐锐敏..马铃薯StKFB29基因克隆、亚细胞定位及其互作蛋白筛选与分析[J].核农学报,2026,40(10):1936-1947,12.基金项目
山西省回国留学人员科研资助项目(2022-094),山西省农业关键核心技术攻关项目(NYGG30-01),马铃薯遗传改良与种质创新山西省重点实验室自主项目(ST202402),中央引导地方科技发展资金项目(YDZJSX2024B007) （2022-094）
