核农学报2026，Vol.40Issue(10)：1948-1959,12.DOI:10.11869/j.issn.1000-8551.2026.10.1948
涝害胁迫下不定根去除对甜瓜叶片生理生化及代谢组的影响
Effects of Adventitious Roots Removal on the Physiological,Biochemical,and Metabolomic Responses of Melon(Cucumis melo L.)Leaves under Waterlogging Stress
摘要
Abstract
Waterlogging is a severe abiotic stress that markedly reduces oxygen availability to submerged organs.Under hypoxic conditions,the hypocotyls of melon(Cucumis melo L.)seedlings induce the formation of adventitious roots.However,the importance of these adventitious roots in melon under waterlogging stress remains largely underexplored.To investigate the role of adventitious roots induced by waterlogging stress in melon seedlings,the physiological,biochemical,and metabolic changes in leaves after artificial removal of these induced adventitious roots were studied.The removal of adventitious roots resulted in abnormal phenotypes,characterized as chlorotic and withered leaves.Compared with the seedlings under normal management and those subjected to waterlogging alone,the removal of adventitious roots led to a highly significant reduction in total chlorophyll content by 63.08％and 43.62％,respectively.Additionally,the removal of adventitious roots significantly elevated the levels of malondialdehyde,H2O2,alcohol dehydrogenase activity,superoxide dismutase activity,and peroxidase activity.Specifically,H2O2 levels increased by 206.87％and 33.81％,while peroxidase activity rose by 289.19％and 20.94％,compared to the normal control and intact waterlogging stress conditions,respectively.Through untargeted metabolomic analysis,a total of 88,119,and 75 differentially accumulated metabolites(DAMs)were identified in WL vs CK,R-AR vs CK and R-AR vs WL,respectively,with eight DAMs shared across all three comparison groups.Notably,peonidin 3-(6″-acetyl-galactoside),R-10-hydroxywarfarin and vitisidin A(annotated as flavonoids),blumenol C glucoside and 16-hydroxyhexadecanoic acid(categorized as lipids),and ingenol(classified as terpenoids),were identified as key metabolites that play important roles in the response to adventitious roots removal under waterlogging stress.This research provides a theoretical basis for elucidating the physiological functions and underlying response mechanisms of adventitious roots under waterlogging stress.关键词
甜瓜/涝害胁迫/不定根/活性氧/代谢组Key words
Cucumis melo/waterlogging stress/adventitious roots/reactive oxygen species/metabolome分类
农业科技引用本文复制引用
张焕欣,闫承璞,陈钱,张哲,李国权..涝害胁迫下不定根去除对甜瓜叶片生理生化及代谢组的影响[J].核农学报,2026,40(10):1948-1959,12.基金项目
江西省农业科学院基础研究与人才培养专项资助项目(JXSNKYJCRC202311),国家自然科学基金项目(32260756),中国博士后科学基金(2021M701514),江西省博士后科研择优资助项目(2021KY44) （JXSNKYJCRC202311）
