核农学报2026，Vol.40Issue(10)：1960-1969,10.DOI:10.11869/j.issn.1000-8551.2026.10.1960
草莓白绢病病原菌鉴定、生物学特性及室内药剂筛选
Identification,Biological Characteristics and Fungicide Screening of the Pathogen Causing Strawberry Southern Blight Disease
摘要
Abstract
To accurately identify the causal agent of strawberry southern blight,clarify its biological characteristics,and screen effective fungicides,strawberry plants with typical southern blight symptoms were collected from Ningbo,Zhejiang.The pathogen was obtained by the tissue isolation method and pathogenicity tests.Based on morphological characteristics,multilocus phylogenetic analysis(ITS-tef-LSU),the pathogen was further confirmed.Then,in vitro fungicide sensitivity assays for 5 fungicides was performed by the mycelial growth rate method.The results showed that,the pathogen causing strawberry southern blight in Ningbo,Zhejiang was Athelia rolfsii,the temperature range for mycelial growth was 15-40 ℃,with an optimum temperature of 30℃,while the most suitable temperature for sclerotia formation was 32℃.The optimum pH for mycelial growth was 5.0-6.5,and the optimal pH for sclerotia formation was 6.0.On modified Czapek medium with different carbon source amendments,sorbitol was the most favorable carbon sources for mycelial growth,while starch and maltose were optimal for sclerotia formation.The optimum nitrogen sources for mycelial growth were beef extract and peptone,whereas for sclerotia formation,KNO3 was the most effective.The temperature range for sclerotia germination was 15-40℃,with an optimum of 30℃.The optimum substrate moisture for sclerotia germination was 60％-90％,and pH value had no significant effect on sclerotia germination.The variability of both mycelia and sclerotia viability was completely eliminated after incubation at 45 ℃ for 10 minutes(hyphal agar plugs)or 52 ℃ for 10 minutes(sclerotia).Laboratory toxicity tests of 5 fungicides showed that Azoxystrobin and fluazinam exhibited the highest inhibitory activity,with EC50 of 0.005 and 0.072 mg·L-1,respectively.While bromothalonil showed the lowest toxicity with an EC50 of 11.885 mg·L-1.To our knowledge,this is the first report of A.rolfsii causing strawberry southern blight in Zhejiang Province.The current study can provide a basis for the scientific management of this disease.关键词
草莓白绢病/室内毒力/罗耳阿太菌/杀菌剂Key words
strawberry southern blight/laboratory toxicity/Athelia rolfsii/fungicide分类
农业科技引用本文复制引用
张国芳,成梦婷,沈岚,于家荣,崔丽利..草莓白绢病病原菌鉴定、生物学特性及室内药剂筛选[J].核农学报,2026,40(10):1960-1969,10.基金项目
宁波市重点研发计划暨"揭榜挂帅"项目(2023Z112),宁波市奉化区科技计划项目(202402107) （2023Z112）
