解剖学杂志2026，Vol.49Issue(4)：326-333,367,9.DOI:10.3969/j.issn.1001-1633.2026.04.003
高强度间歇训练通过乳酸/GPR81/ERK信号通路促进小鼠大脑皮质血管生成
High-intensity interval training promotes angiogenesis in the cerebral cortex of mice through the lactate/GPR81/ERK signaling pathway
摘要
Abstract
Objective:To investigate whether high-intensity interval training(HIIT)promotes cerebral cortical angiogenesis in mice through the lactate/G-protein-coupled receptor 81(GPR81)/extracellular signal-regulated kinase(ERK)signaling pathway.Methods:Nine-week-old C57BL/6J mice were randomly assigned to a control group or a HIIT group subjected to a 6-week training regimen.Mouse cerebral vascular endothelial cells(bEnd.3)were divided to control group,lactate group,lactate+ERK inhibitor group,GPR81 agonist group and GPR81 agonist+ERK inhibitor group.Cortical microvascular density and length were evaluated by immunofluorescence staining.Protein expression of collagen Ⅳ,GPR81,total ERK,and phosphorylated ERK(p-ERK)was analyzed by Western blotting.Cell migration,proliferation,and tube-forming ability were assessed by wound healing,CCK-8,and Matrigel tube formation assays,respectively.Results:A single bout of HIIT significantly elevated blood lactate levels.After the 6-week intervention,HIIT enhanced cortical angiogenesis,as indicated by increased microvascular density and length,and upregulation of collagen Ⅳ,GPR81,and p-ERK expression.In vitro,both lactate and GPR81 activation enhanced bEnd.3 cell proliferation,migration,and tube formation.Notably,ERK inhibition attenuated the lactate-and GPR81 agonist-induced enhancement of these cellular functions.Conclusion:HIIT induces angiogenesis in the mouse cerebral cortex through the lactate/GPR81/ERK signaling pathway.关键词
高强度间歇训练/大脑皮质/血管生成/乳酸/G蛋白偶联受体81/细胞外调节蛋白激酶Key words
high-intensity interval training/cerebral cortex/angiogenesis/lactate/G-protein-coupled receptor 81/extracellular signal-regulated protein kinase分类
医药卫生引用本文复制引用
童玥,卞学鹏,邓梦婷,商庆慧,刘俊,祝露桃,娄淑杰..高强度间歇训练通过乳酸/GPR81/ERK信号通路促进小鼠大脑皮质血管生成[J].解剖学杂志,2026,49(4):326-333,367,9.基金项目
国家自然科学基金(31971098) （31971098）
上海市人类运动能力开发与保障重点实验室(上海体育大学)资助项目(11DZ2261100) （上海体育大学）
