解剖学杂志2026，Vol.49Issue(4)：355-361,7.DOI:10.3969/j.issn.1001-1633.2026.04.008
LINC00852调节miR-671-5p/SETD5轴对食管鳞状细胞癌细胞恶性进展的影响
Effects of LINC00852 on malignant progression of esophageal squamous cell carcinoma cells by regulating the miR-671-5p/SETD5 axis
摘要
Abstract
Objective:To investigate the effect of long noncoding RNA00852(LINC00852)on the malignant progression of esophageal squamous cell carcinoma(ESCC)cells by regulating the microRNA-671-5p(miR-671-5p)/SET domain-containing protein 5(SETD5)axis.Methods:RT-qPCR was used to measure the expression levels of LINC00852,miR-671-5p,and SETD5 mRNA in the ESCC tissues and adjacent non-tumor tissues from 56 ESCC patients who underwent surgery in our hospital from March 2023 to March 2024.Human esophageal squamous cell carcinoma cell line TE11 cells were selected as research objects and divided into five groups:Control,sh-NC,sh-LINC00852,sh-LINC00852+anti-NC,and sh-LINC00852+anti-miR-671-5p.EdU staining,Transwell assay,and flow cytometry were applied to detect the effects of LINC00852 silencing on the proliferation,migration,invasion,and apoptosis of TE11 cells.Western blotting was employed to measure the expression of PCNA,SETD5,MMP-9,and Bax proteins in TE11 cells.Dual-luciferase reporter gene assay was utilized to determine the interaction between LINC00852 and miR-671-5p,and between miR-671-5p and SETD5.Results:The expression of LINC00852 and SETD5 mRNA in ESCC tissues was higher than that in adjacent non-tumor tissues,while the expression of miR-671-5p was lower.The EdU-positive cell rate,migration and invasion numbers,LINC00852,SETD5 mRNA and protein levels,PCNA and MMP-9 protein levels in the sh-LINC00852 group were lower than those in the sh-NC group and the Control group,while the apoptosis rate,miR-671-5p,and Bax protein levels were higher than those in the sh-NC group and the Control group.Compared with the sh-LINC00852 group and the sh-LINC00852+anti-NC group,the sh-LINC00852+anti-miR-671-5p group showed increased EdU-positive cell rate,migration and invasion numbers,SETD5 mRNA and protein levels,PCNA and MMP-9 protein expression,but decreased apoptosis rate,miR-671-5p expression,and Bax protein expression.Conclusion:LINC00852 promotes the malignant progression of ESCC cells by regulating the miR-671-5p/SETD5 axis.关键词
长链非编码RNA00852/微小RNA-671-5p/含SET结构域蛋白5/食管鳞状细胞癌/恶性进展Key words
long noncoding RNA00852/miR-671-5p/SET domain-containing protein 5/esophageal squamous cell carcinoma/malignant progression分类
医药卫生引用本文复制引用
许芬芬,刘劼,熊玉梅,郭剑锋,谢玉芳..LINC00852调节miR-671-5p/SETD5轴对食管鳞状细胞癌细胞恶性进展的影响[J].解剖学杂志,2026,49(4):355-361,7.基金项目
攀枝花市2022年度市级指导性科技计划项目(2022ZD-S-48) （2022ZD-S-48）
