扬州大学学报（农业与生命科学版）2026，Vol.47Issue(4)：14-21,8.DOI:10.16872/j.cnki.1671-4652.2026.04.002
海门山羊SOCS2基因克隆、生物信息学分析及单碱基编辑的研究
Study of cloning,bioinformatics analysis and base editing of SOCS2 gene in Haimen goat
摘要
Abstract
To verify the applicability of base editing technology in Haimen goat fibroblasts,this study first extracted RNA from the longissimus dorsi muscle tissue of Haimen goats,amplified and cloned the suppressor of cytokine signaling 2 gene(SOCS2)sequence via RT-PCR,subsequently performed bioinformatics analysis of the sequence using online tools,and detected the expression levels of the SOCS2 gene in multiple tissues by quantitative real-time PCR.Two sgRNAs tar-geting the second exon region of the SOCS2 gene were designed and ligated into the sgRNA expression vector plasmids,which were then co-transfected with the YE1-BE3-FNLS plasmid into goat fetal fibroblasts.Gene editing was assessed by Sanger sequencing and T-A cloning.The results showed that the cloned coding sequence of the SOCS2 gene from Haimen goats was 856 bp in length,with a CDS region of 597 bp encoding 198 amino acids.The SOCS2 gene exhibited the high-est expression level in the liver tissue of Haimen goats.The single-base editing efficiency of the sgRNA reached 31.58％.In summary,this study successfully cloned the SOCS2 gene of Haimen goats and achieved single-base editing in the cod-ing region of the SOCS2 gene in goat fetal fibroblasts,laying a foundation for the use of single-base gene editing technolo-gy in breeding new meat-type Haimen goat varieties.关键词
海门山羊/细胞因子信号传导抑制因子2/单碱基编辑Key words
Haimen goat/suppressor of cytokine signaling 2/single base editing分类
农业科技引用本文复制引用
白冀昀,李玲,EJAZ Ahmad,TARIQ Sohail,MUHAMMAD Farhab,宋志贤,陈风雷,薛刚,袁玉国..海门山羊SOCS2基因克隆、生物信息学分析及单碱基编辑的研究[J].扬州大学学报（农业与生命科学版）,2026,47(4):14-21,8.基金项目
江苏省种业振兴"揭榜挂帅"项目[JBGS(2021)025] （2021）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
高等学校学科创新引智计划资助项目(D18007) （D18007）
扬州市市校合作专项(YZ2023205) （YZ2023205）
江苏省研究生实践创新计划项目(SJCX25_2376) （SJCX25_2376）
