扬州大学学报（农业与生命科学版）2026，Vol.47Issue(4)：22-33,12.DOI:10.16872/j.cnki.1671-4652.2026.04.003
1株高致病性样猪繁殖与呼吸综合征病毒株的分离及致病性分析
Isolation and pathogenicity analysis of a highly pathogenic-like porcine reproductive and respiratory syndrome virus(HP-like PRRSV)
摘要
Abstract
To investigate the genetic variation and pathogenic mechanisms of porcine reproductive and respiratory syn-drome virus(PRRSV)strains currently circulating in China,this study isolated and purified a PRRSV strain,designated HUN/2022/F2,from lung samples of pigs exhibiting suspected clinical symptoms using Marc-145 cells.The genetic rela-tionship of the isolate was determined via whole-genome sequencing and phylogenetic analysis.Furthermore,its pathoge-nicity was comprehensively evaluated by inoculating PRRSV-naive piglets and monitoring clinical scores,growth perform-ance,viral loads,and histopathological changes.Genomic analysis revealed that the HUN/2022/F2 strain clusters within lineage 8.7 and shared a close genetic relationship with the highly pathogenic PRRSV(HP-PRRSV)strains prevalent in China,identifying it as an HP-like PRRSV variant.In the challenge experiment,infected piglets exhibited typical clinical symptoms,including persistent high fever(≥40.0℃),anorexia,dyspnea,and hind limb paralysis,accompanied by a significant reduction in average daily gain.Viremia persisted for over two weeks,with high levels of viral replication detected primarily in the lungs and tonsils.Histopathological examination demonstrated extensive interstitial pneumonia character-ized by thickened alveolar septa and inflammatory cell infiltration,as well as hemorrhage and diffuse lymphocytic hyper-plasia in the lymph nodes.In conclusion,the isolate HUN/2022/F2 is an HP-like PRRSV strain that exhibits pathogenic-ity in piglets,causing typical respiratory distress and tissue damage.These findings enrich the genetic database of PRRSV strains in China and provide a scientific basis for the epidemiological surveillance and control of the disease.关键词
猪繁殖与呼吸综合征病毒/病毒分离/致病性Key words
porcine reproductive and respiratory syndrome virus/virus isolation/pathogenicity分类
农业科技引用本文复制引用
卓晨,赵兴,牟春晓,严玉霖,陈振海..1株高致病性样猪繁殖与呼吸综合征病毒株的分离及致病性分析[J].扬州大学学报（农业与生命科学版）,2026,47(4):22-33,12.基金项目
撒坝猪健康养殖关键技术研究与应用项目(202502AE09059) （202502AE09059）
扬州大学"青蓝工程"资助项目[YDRS(2024)-22] （2024）
江苏高校优势学科建设工程资助项目(PAPD) （PAPD）
