扬州大学学报（农业与生命科学版）2026，Vol.47Issue(4)：34-43,10.DOI:10.16872/j.cnki.1671-4652.2026.04.004
火鸡组织滴虫强毒虫株与其传代致弱虫株的比较转录组学分析
Comparative transcriptomic analysis between virulent and passage-attenuated strains of Histomonas meleagridis
摘要
Abstract
To investigate the molecular mechanisms underlying the attenuation of Histomonas meleagridis,we conduc-ted a comparative analysis of an attenuated strain(JSYZ-D168)and a virulent strain(JSYZ-D10)using animal experi-ments combined with transcriptome sequencing.The results showed that the pathogenicity of D168 strain was significant-ly reduced and conferred a degree of immune protection in chickens.A total of 1 160 differentially expressed genes(DEGs)were identified between D10 and D168,with 188 genes upregulated and 972 downregulated in D10.Gene ontolo-gy(GO)enrichment analysis revealed that DEGs were significantly enriched in chromosome,histone lysine N-methyl-transferase activity,glucose homeostasis,cell-cell adhesion,and membrane components.Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis indicated that DEGs were significantly associated with the NOD-like re-ceptor signaling pathway,inflammatory mediator regulation of TRP channels,and the Toll and Imd signaling pathways.Eight DEGs were randomly selected for qRT-PCR validation,and the results were consistent with the transcriptome data,confirming the accuracy and reliability of the sequencing results.In conclusion,this study provides a theoretical basis for the development of a live-attenuated vaccine against H.meleagridis.关键词
火鸡组织滴虫/致弱/转录组测序/差异表达基因Key words
Histomonas meleagridis/attenuation/transcriptome sequencing/differentially expressed genes分类
农业科技引用本文复制引用
刘亚新,陈乔光,祝文迪,候照峰,刘丹丹,陶建平,许金俊..火鸡组织滴虫强毒虫株与其传代致弱虫株的比较转录组学分析[J].扬州大学学报（农业与生命科学版）,2026,47(4):34-43,10.基金项目
国家自然科学基金面上项目(31772727) （31772727）
扬州市重点研发计划(现代农业)项目(YZ2017051) （现代农业）
江苏高校优势学科建设三期工程资助项目(PAPD) （PAPD）
