扬州大学学报（农业与生命科学版）2026，Vol.47Issue(4)：44-53,10.DOI:10.16872/j.cnki.1671-4652.2026.04.005
黄曲霉毒素B1对罗氏沼虾生长性能、免疫、抗氧化能力和肝胰腺健康的影响
The effects of aflatoxin B1 on growth performance,immunity,antioxidant capacity and hepatopancreatic health of giant river prawn(Macrobrachium rosenbergii)
摘要
Abstract
This study aimed to investigate the effects of aflatoxin B1(AFB1)on growth performance,immune function,antioxidant capacity and hepatopancreatic health of giant freshwater prawn,Macrobrachium rosenbergii.A 60-day feeding trial was conducted by supplementing basal diets with different levels of AFB1,establishing four groups:CON group,AFB1-20 group,AFB1-100 group and AFB1-500 group,with AFB1 supplementation levels of 0,20,100 and 500 μg·kg-1,respectively.The results showed that as dietary AFB1 level increased the growth performance of M.rosenbergii significant-ly decreased,characterized by significantly reduced final body weight,weight gain rate,specific growth rate and survival rate,and a significantly increased feed conversion ratio.Meanwhile,the activities of hepatopancreatic digestive enzymes,including trypsin and amylase,were significantly decreased.Serum biochemical analysis showed that the activities of ALT,iNOS,ACP and AKP were significantly increased in the AFB1-treated groups.The activities of SOD and GSH-Px in the hepatopancreas were significantly decreased;when the dietary AFB1 inclusion level reached 100 μg·kg-1 or high-er,GST activity was significantly decreased,whereas MDA content was significantly increased.Meanwhile,the relative expression levels of immune-related genes,including imd,relish and toll,were significantly downregulated in all AFB1-treated groups,whereas the relative expression levels of MrIAP and peroxiredoxinr-5 were significantly upregulated in the AFB1-500 group.Regarding apoptosis,the relative expression levels of the pro-apoptotic genes bok,caspase 3 and caspase 9 in the hepatopancreas of the AFB1-500 group were significantly higher than those in the CON group.In conclu-sion,AFB1 contamination significantly reduced growth performance and digestive capacity,impaired hepatopancreatic an-tioxidant and immune functions,and promoted apoptosis in M.rosenbergii,thereby adversely affecting prawn health.Therefore,raw material screening,storage management,and feed testing should be strengthened in aquaculture produc-tion to prevent AFB1-contaminated feed from being used in M.rosenbergii farming.关键词
罗氏沼虾/黄曲霉毒素B1/生长性能/抗氧化能力/肝胰腺健康Key words
Macrobrachium rosenbergii/aflatoxin B1/growth performance/antioxidant capacity/hepatopancreatic health分类
农业科技引用本文复制引用
王安然,郭祥基,徐洁,顾伟,张鑫,邓康裕,邓登,苗淑彦..黄曲霉毒素B1对罗氏沼虾生长性能、免疫、抗氧化能力和肝胰腺健康的影响[J].扬州大学学报（农业与生命科学版）,2026,47(4):44-53,10.基金项目
江苏省农业重大技术协同推广计划项目(2023-ZYXT-10) （2023-ZYXT-10）
