扬州大学学报（农业与生命科学版）2026，Vol.47Issue(4)：54-65,12.DOI:10.16872/j.cnki.1671-4652.2026.04.006
大麦赤霉病抗源筛选及抗性关联分析
Screening of germplasm and association analysis for resistance to Fusarium head blight in barley
摘要
Abstract
In this study,101 barley varieties(lines)were used to evaluate the Fusarium head blight(FHB)resistance through a combination of artificial single-floret drop inoculation and natural infection methods.Meanwhile,genotype anal-ysis of the test materials was conducted using simple sequence repeat(SSR)markers combined with resistance identifica-tion,and an association analysis was conducted to identify FHB resistance loci in barley.The results showed that the av-erage diseased spikelet rate of the 101 test materials at the Yangzhou and Nanping test site were 20.65％and 21.12％,respectively.A total of 49 materials achieved moderate resistance(MR)or resistance(R)levels to FHB at both test sites.Using 87 pairs of SSR markers,479 alleles were detected in the 101 test materials,with an average polymorphism information content(PIC)of 0.63.Population structure analysis divided the test materials into 6 subpopulations.Associ-ation analysis indicated that 11 and 8 markers were significantly associated with FHB resistance at the Yangzhou and Nan-ping sites,respectively(P＜0.05).Among these,four markers(Bmag0140,Bmag0877,Bmag0387,and Ebmac0806)were consistently detected at both sites.Specifically,the marker Bmag0140 had a relatively high explanation rate for phe-notypic variation at the two sites(12.22％and 9.88％),and it might be associated with resistance to deoxynivalenol(DON)toxin accumulation.In addition,Bmag0877,Bmag0387,and Ebmac0806 were also significantly associated with agronomic traits such as plant height and spike length.This study provides a valuable information for molecular marker-assisted selection in barley breeding for FHB resistance.关键词
大麦/赤霉病/种质筛选/关联分析/SSR标记/抗性育种Key words
barley/Fusarium head blight(FHB)/germplasm screening/association analysis/SSR markers/resist-ance breeding分类
农业科技引用本文复制引用
任圆媛,石玲,郭宝健,王菲菲,朱娟,许如根,吕超..大麦赤霉病抗源筛选及抗性关联分析[J].扬州大学学报（农业与生命科学版）,2026,47(4):54-65,12.基金项目
国家大麦青稞产业技术体系建设专项(CARS-05) （CARS-05）
江苏省高校优势学科建设工程资助项目(PAPD) （PAPD）
