扬州大学学报（农业与生命科学版）2026，Vol.47Issue(4)：89-97,126,10.DOI:10.16872/j.cnki.1671-4652.2026.04.009
瓦尼桑黄黄酮提取工艺优化及抗氧化活性分析
Optimization of extraction process of flavonoids from Sanghuangporus vaninii and its antioxidant activity determination
摘要
Abstract
Using a high-flavonoid-producing strain of Sanghuangporus vaninii,this study aimed to optimize the ultra-sonic-assisted ethanol extraction process for flavonoids(ST-CAS).Single factor experiments,Plackett-Burman design,and response surface methodology(RSM)were employed to identify the most significant factors influencing the extraction of ST-CAS,optimize the ultrasound-assisted ethanol extraction process and evaluate the antioxidant activity of ST-CAS using DPPH and ABTS+assays.The results showed that the optimal ultrasound assisted ethanol extraction process for ST-CAS in the dry mycelium of S.vaninii was as follows:solid-liquid ratio(g∶mL)1∶36,ethanol concentration 65％and extraction temperature 66℃.Under these process conditions,the extraction yield of ST-CAS was(33.18±0.09)mg·g-1.ST-CAS exhibited significant scavenging activity against DPPH and ABTS+,with half-maximal inhibitory concentrations(IC50)of 0.353 and 0.203 mg·mL-1,respectively,and scavenging rates of 92.58％and 98.72％.In summary,the re-sponse surface methodology employed to optimize the ultrasound-assisted ethanol extraction process of ST-CAS is relia-ble,and ST-CAS exhibits strong antioxidant activity.This study provides a theoretical basis for the extraction,develop-ment and utilization of ST-CAS.关键词
瓦尼桑黄/黄酮/超声辅助醇提法/响应面法/抗氧化活性Key words
Sanghuangporus vaninii/flavonoids/ultrasound-assisted ethanol extraction/response surface method/antioxidant activity分类
农业科技引用本文复制引用
石晶晶,陆源添,刘迪..瓦尼桑黄黄酮提取工艺优化及抗氧化活性分析[J].扬州大学学报（农业与生命科学版）,2026,47(4):89-97,126,10.基金项目
国家重点研发计划项目子项目(2017YFD0300104-02) （2017YFD0300104-02）
中韩国际农业技术合作项目(延大横向[2019]第19号) （延大横向[2019]第19号）
延边大学校企合作项目(延大科合字[2020]2号) （延大科合字[2020]2号）
