扬州大学学报（农业与生命科学版）2026，Vol.47Issue(4)：98-106,9.DOI:10.16872/j.cnki.1671-4652.2026.04.010
基于H2DCFDA标记及NMT技术研究海雀稗根-叶H2O2信号的长距离传递现象
Investigation of long-distance transmission of root-leaf H2O2 signaling in Paspalum vaginatum based on H2DCFDA labeling and non-invasive micro-test technology(NMT)
摘要
Abstract
H2O2 signals between roots and leaves can uncover the long-distance communication mechanism underlying plant systemic responses to environmental stresses.To explore whether there is a long-distance transport of H2O2 signals between roots and leaves of(Paspalum vaginatum),an experimental system for root-derived and leaf-derived H2O2 sig-nals was established.The NADPH oxidase inhibitor diphenyleneiodonium chloride(DPI)was applied,combined with H2DCFDA fluorescent probe tracing,non-invasive micro-test technology(NMT)and automatic colorimetric plant pheno-typing analysis(ACA),the root-and leaf-originated H2O2 induced by salt stress and transient heat shock were tracked and investigated,confirming the bidirectional long-distance transmission of H2O2 signals in Paspalum vaginatum.The re-sults showed that root stress with 200 mmol·L-1 NaCl combined with stem treatment of 20 pmol·L-1 DPI successfully constructed a root-derived H2O2 signal deletion system.Root-originated H2O2 could be transported to leaves over a long distance under salt stress,while DPI treatment extremely significantly inhibited its translocation and interfered with intra-and extrachloroplastic H2O2 signal transmission.Transient heat shock at 43℃ for 1-2 h only caused mild stress and could stably induce the generation of leaf-derived H2O2;heat shock-induced leaf H2O2 could be reversibly transported to roots,and inhibition of leaf H2O2 production by DPI led to an extremely significant decrease in fluorescence signal at root tips.This study demonstrates that bidirectional long-distance transmission of root-and leaf-derived H2O2 occurs in Pas-palum vaginatum.The findings provide a scientific basis for further exploring the transmission of abiotic stress-induced H2O2 signals and their regulatory mechanisms underlying abiotic stress tolerance in plants.关键词
海雀稗/H2O2信号/长距离传递/双向传递/非生物胁迫Key words
Paspalum vaginatum/hydrogen peroxide signal/long-distance transmission/bidirectional transmission/abiotic stress分类
农业科技引用本文复制引用
彭迪,汤青松,陆洋,潘玲..基于H2DCFDA标记及NMT技术研究海雀稗根-叶H2O2信号的长距离传递现象[J].扬州大学学报（农业与生命科学版）,2026,47(4):98-106,9.基金项目
国家自然科学基金资助项目(32401488) （32401488）
