菌物学报2026，Vol.45Issue(9)：118-128,11.DOI:10.13346/j.mycosystema.260053
皱环球盖菇锰过氧化物酶基因家族及其在不同农业废弃物培养基上的表达
Manganese peroxidase gene family in Stropharia rugosoannulata and its expression on various agricultural waste substrates
摘要
Abstract
The manganese peroxidase(MnP)gene family plays a crucial role in the extracellular degradation of lignocellulose by white-rot fungi.In this study,the MnP gene family in the genome of Stropharia rugosoannulata was systematically identified and analyzed using bioinformatics approaches,and the effects of different agricultural wastes(T1-T5)on mycelial growth,MnP activity,and the relative expression levels of MnP gene family members were investigated.The results showed that eight MnP gene family members(SrMnP1-SrMnP8)were identified from the S.rugosoannulata genome,and they were distributed at six chromosomes.Phylogenetic analysis revealed species-specific clades.The addition of 2%cottonseed hull(T3)and 4%sawdust(T5)significantly promoted mycelial growth.The T5 treatment showed the highest mycelial dry weight,increasing 44 times as compared with the control.MnP activity was significantly increased in the sawdust treatments,with the highest enzyme activities observed in the 2%sawdust(T4)and 4%sawdust(T5)treatments,reaching 4.85 U/g and 4.25 U/g,respectively.Gene expression analysis revealed that SrMnP3 exhibited significantly higher expression levels as compared with other SrMnPs across all treatments.This study preliminarily revealed the composition and expression characteristics of the MnP gene family in S.rugosoannulata,defining SrMnP3 as a key player in lignocellulose degradation.These findings provide technical references for optimizing fungal degradation capabilities through molecular approaches and enhancing the resource utilization of agricultural and forestry residues.关键词
皱环球盖菇/锰过氧化物酶/农业废弃物/相对表达量Key words
Stropharia rugosoannulata/manganese peroxidase/agricultural waste/relative expression level引用本文复制引用
李佳书,李念祖,张薇薇,由书宁,徐定高,张国庆..皱环球盖菇锰过氧化物酶基因家族及其在不同农业废弃物培养基上的表达[J].菌物学报,2026,45(9):118-128,11.基金项目
北京市自然科学基金(6232003) （6232003）
现代农业产业技术体系北京市食用菌创新团队(BAIC03) This work was supported by the Beijing Natural Science Foundation(6232003)and the Beijing Innovation Consortium of Agriculture Research System(BAIC03). （BAIC03）
