检验医学与临床2026，Vol.23Issue(17)：2332-2337,2344,7.DOI:10.3969/j.issn.1672-9455.2026.17.005
血清miR-124-3p和miR-214-3p对年龄相关性白内障的诊断价值
Diagnostic value of serum miR-124-3p and miR-214-3p in age-related cataract
摘要
Abstract
Objective To investigate the diagnostic value of serum miR-124-3p and miR-214-3p levels in age-related cataract(ARC).Methods A total of 98 ARC patients who were treated in Xining Aier Eye Hospi-tal from January 2022 to December 2023 were selected as the study group.Another 95 healthy people who un-derwent physical examination in Xining Aier Eye Hospital during the same period were selected as the control group.The serum levels of miR-124-3p and miR-214-3p in all subjects were detected by real-time fluorescence quantitative polymerase chain reaction.The serum levels of superoxide dismutase(SOD),glutathione peroxi-dase(GSH-Px)and malondialdehyde(MDA)were detected by enzyme-linked immunosorbent assay in all subjects.Baseline data of all subjects were collected.Pearson correlation was used to analyze the correlation between serum miR-124-3p,miR-214-3p and SOD,MDA,GSH-Px levels in ARC patients.Multivariate Logis-tic regression was used to analyze the influencing factors of ARC.The receiver operating characteristic(ROC)curve was drawn to analyze the diagnostic value of serum miR-124-3p,miR-214-3p alone and combined detec-tion for ARC.Results The serum MDA level of the study group was higher than that of the control group,and the serum SOD,GSH-Px,miR-124-3p and miR-214-3p levels were lower than those of the control group,and the differences were statistically significant(P＜0.05).Pearson correlation analysis showed that serum miR-124-3p level in ARC patients was positively correlated with serum SOD and GSH-Px levels(P＜0.05),and negatively correlated with serum MDA level(P＜0.05).The level of serum miR-214-3p was positively correlated with the levels of SOD and GSH-Px(P＜0.05),and negatively correlated with the level of serum MDA(P＜0.05).The results of multivariate Logistic regression analysis showed that the increase of serum SOD,GSH-Px,miR-124-3p and miR-214-3p levels were protective factors for ARC(P＜0.05),and the in-crease of serum MDA level was a risk factor for ARC(P＜0.05).The area under the curve(AUC)of serum miR-124-3p,miR-214-3p alone and combined detection in the diagnosis of ARC was 0.756,0.770 and 0.837,respectively.The AUC of the combined detection in the diagnosis of miR-124-3p and miR-214-3p was greater than that of miR-124-3p or miR-214-3p alone(Z=1.782,1.525,P＜0.05).Conclusion The levels of serum miR-124-3p and miR-214-3p in ARC patients are decreased,and they are involved in the occurrence and devel-opment of ARC.The combination of miR-124-3p and miR-214-3p has certain diagnostic value for ARC.关键词
年龄相关性白内障/miR-124-3p/miR-214-3p/超氧化物歧化酶/谷胱氨酸过氧化物酶/丙二醛Key words
age-related cataract/miR-124-3p/miR-214-3p/superoxide dismutase/glutathione per-oxidase/malondialdehyde分类
医药卫生引用本文复制引用
张道远,张琼,李磊..血清miR-124-3p和miR-214-3p对年龄相关性白内障的诊断价值[J].检验医学与临床,2026,23(17):2332-2337,2344,7.基金项目
青海省卫生健康系统重点课题(2023-wizd-12). （2023-wizd-12）
