辽宁农业科学Issue(5)：16-21,6.DOI:10.3969/j.issn.1002-1728.2026.05.003
玉米抗大斑病Ht3L基因的精细定位及育种应用评价
Fine Mapping of the Ht3-like Gene for Northern Corn Leaf Blight Resistance in Maize and Evaluation of Its Breeding Application
摘要
Abstract
Northern corn leaf blight(NCLB)is a major foliar disease threatening maize production.In this study,using a map-based cloning strategy,we fine-mapped the previously identified major quantitative resist-ance locus Ht3L to 317 kb physical interval on chromosome 8.A total of 15 annotated genes were identified in this region,and ZmWAK-RLK1 and ZmWAK-RLK2 were identified as the primary candidate genes.Mean-while,through backcrossing and marker-assisted selection,Ht3L was introgressed into the susceptible elite in-bred line Liao 3162,generating a series of improved resistant lines and their isohybrids.Results of disease re-sistance and yield evaluation at the Shenyang trial site showed that the improved inbred lines carrying Ht3L ex-hibited a significantly reduced diseased leaf area(DLA)compared with the recurrent parent Liao 3162(P<0.05),along with a significant increase in yield.The corresponding hybrids also effectively reduced DLA while showing no significant changes in major agronomic traits such as ear length,ear diameter,plant height,and ear height,and their yield was notably improved.Ht3L is an elite gene locus conferring durable disease resistance and high-yield potential,and it holds important application value for NCLB resistance breeding in the spring maize region of Northeast China.关键词
玉米大斑病/Ht3L基因/分子标记辅助选择/育种应用Key words
Northern corn leaf blight/Ht3L gene/Molecular marker-assisted selection/Application in breed-ing分类
农业科技引用本文复制引用
齐欣,姜敏,贾钰莹,张默,刘欣芳,马骏..玉米抗大斑病Ht3L基因的精细定位及育种应用评价[J].辽宁农业科学,2026,(5):16-21,6.基金项目
国家重点研发计划项目(2021YFD1200701-3) （2021YFD1200701-3）
