辽宁农业科学Issue(5)：49-53,5.DOI:10.3969/j.issn.1002-1728.2026.05.008
番茄SlBEL9基因生物信息学及表达模式分析
Bioinformatics and Expression Pattern Analysis of SlBEL9 Gene in Solanum lycopersicum L.
摘要
Abstract
In previous study,the transcription factor SlBEL9,which closely related to chlorophyll content in tomato fruits,was screened from the transcriptome data.The function of the gene was predicted by using bioinformatics and qRT-PCR methods.The results showed that the open reading frame of SlBEL9 gene was 1515 bp.It encoding 504 amino acids harboring typical POX and Homeodomain conserved domains.Molecu-lar evolution analysis revealed that SlBEL9 was closely related to the homologous protein of Solanum verruco-sum and Solanum tuberosum,and the homology is 90.80%and 90.59%,respectively.Subcellular localization prediction suggested that the protein was most likely located in the nucleus.Protein-protein interaction analysis showed that SlBEL9 interacted with TKN2 and TKN2 proteins.qRT-PCR analysis revealed that the relative ex-pression levels of SlBEL9 was the highest in green mature fruit and the lowest in the breaker stage.The ex-pression of SlBEL9 first increased and then decreased during the process of fruit development,which was con-sistent with the change of chlorophyll content in the fruit.Therefore,it is speculated that SlBEL9 may play a role upstream of SlTKN2 and SlTKN4,and positively regulates the synthesis of chlorophyll in tomato fruits.关键词
茄/SlBEL9/生物信息学/表达模式分析Key words
Tomato/SlBEL9/Bioinformatics/Expression pattern analysis分类
农业科技引用本文复制引用
王涛,张子君,张逸鸣,吴媛媛,邹庆道..番茄SlBEL9基因生物信息学及表达模式分析[J].辽宁农业科学,2026,(5):49-53,5.基金项目
辽宁省农业科学院协同创新专项(2024XTCX0402) （2024XTCX0402）
兵团科技计划重点领域科技攻关项目(2025AB087) （2025AB087）
沈阳市种业创新专项(24-215-2-10) （24-215-2-10）
