现代畜牧兽医Issue(8)：8-12,5.DOI:10.20154/j.cnki.issn1672-9692.2026.08.002
温度分段调控优化固态发酵饲用纤维素酶的研究
Study on optimizing solid-state fermentation of feed cellulase by segmented temperature regulation
摘要
Abstract
To break the bottleneck that single-temperature solid-state fermentation restricts the cellulase production potential of Aspergillus niger,this study screened the duration and optimal temperature for the vegetative growth phase,cellulase production phase,and cellulase accumulation phase of Aspergillus niger via single-factor experiments and response surface methodology,based on the stage-specific physiological and metabolic differences of the strain.The results demonstrated that the globally optimal process parameters for solid-state fermentation of Aspergillus niger under segmented temperature control were as follows:within a total fermentation cycle of 144 h,the temperature was maintained at 30℃during the vegetative growth phase(0 to 64 h),25℃during the cellulase synthesis phase(65 to 104 h),and 23℃during the cellulase accumulation phase(105 to 144 h).Under these optimized conditions,the cellulase activity produced by Aspergillus niger increased by 67.9%compared with the pre-optimization control.This study verifies that segmented temperature regulation during solid-state fermentation of Aspergillus niger can effectively enhance its cellulase production activity.关键词
黑曲霉/纤维素酶/固态发酵/响应面试验Key words
Aspergillus niger/Cellulase/Solid-state fermentation/Response surface methodology分类
农业科技引用本文复制引用
刘建峰..温度分段调控优化固态发酵饲用纤维素酶的研究[J].现代畜牧兽医,2026,(8):8-12,5.基金项目
湖北省重点实验室开放基金(EWPL201709) （EWPL201709）
