现代畜牧兽医Issue(8)：17-22,6.DOI:10.20154/j.cnki.issn1672-9692.2026.08.004
葡萄籽皂苷的提取工艺优化及其体外抗氧化性研究
Optimization of extraction process for saponins from grape seeds and their in vitro antioxidant activity
摘要
Abstract
To optimize the efficient extraction process of saponins from grape seeds and evaluate their in vitro antioxidant activity,ultrasonic-assisted extraction was adopted in this study.Four variables including ethanol concentration,ultrasonic time,ultrasonic temperature and liquid-solid ratio were selected as investigative factors.Single-factor experiments were first performed to determine the suitable range of each factor,followed by process optimization via response surface methodology.Meanwhile,the in vitro antioxidant capacity of grape seed saponins was assessed by measuring the scavenging rates of DPPH free radicals and hydroxyl free radicals.The results demonstrated that the optimal extraction conditions for grape seed saponins were as follows:Ethanol concentration of 61%,ultrasonic treatment time of 28 min,liquid-solid ratio of 10 mL/g,and ultrasonic temperature of 43℃.Under these optimized conditions,the scavenging rate of the grape seed saponins against DPPH free radicals reached 57.02%,while the scavenging rate against hydroxyl free radicals was 26.46%.This study verified that response surface methodology is scientific and feasible for optimizing the ultrasonic-assisted extraction process of grape seed saponins.The obtained saponin extract exhibits favorable in vitro antioxidant activity,which can provide a theoretical reference for the efficient utilization of functional components in grape seeds and related product development.关键词
葡萄籽/超声提取/皂苷/抗氧化Key words
Grape seed/Ultrasonic extraction/Saponins/Antioxidant activity分类
农业科技引用本文复制引用
侯文静,刘宁,潘春梅..葡萄籽皂苷的提取工艺优化及其体外抗氧化性研究[J].现代畜牧兽医,2026,(8):17-22,6.基金项目
河南省重大科技专项(181100211400) （181100211400）
河南牧业经济学院食品科学与工程重点学科建设项目(XJXK202203) （XJXK202203）
河南省科技攻关项目(242102111018) （242102111018）
