现代畜牧兽医Issue(8)：29-36,8.DOI:10.20154/j.cnki.issn1672-9692.2026.08.006
基于网络药理学及毒理学筛选百部抗弓形虫关键作用成分及验证
Screening and verification of key active components of Stemonae Radix against Toxoplasma gondii based on network pharmacology and toxicology
摘要
Abstract
To screen and verify the key active ingredients and targets of the traditional Chinese medicine Stemonae Radix against Toxoplasma gondii,this study adopted network pharmacology and network toxicology methods to identify its main active components,potential toxicity and effector targets against Toxoplasma gondii,and further analyze the cytotoxicity of the principal active ingredients as well as their inhibitory effects on Toxoplasma gondii.The results showed that network pharmacology identified more than ten main active components such as β-sitosterol and oleanolic acid,and the key targets for anti-Toxoplasma gondii were PPARG,SIRT1,PTGS2,JUN,BCL2,etc;network toxicology revealed that more than half of the components had reproductive toxicity,and some had respiratory toxicity and ocular irritation;the main active components of Stemonae Radix,β-sitosterol and oleanolic acid,had no cytotoxicity and could significantly inhibit the invasion and proliferation of Toxoplasma gondii.The study demonstrated that the main active components of Baibu identified through network pharmacology,β-sitosterol and oleanolic acid,did not cause cytotoxicity and could significantly inhibit the invasion and proliferation process of Toxoplasma gondii.关键词
百部/网络药理学/弓形虫/网络毒理学Key words
Stemonae Radix/Network Pharmacology/Toxoplasma gondii/Network Toxicology分类
农业科技引用本文复制引用
刘澈,甘盛琦,施玉怡,宋紫沁,杨宝玲,刘艳霞,王秀明,王大为..基于网络药理学及毒理学筛选百部抗弓形虫关键作用成分及验证[J].现代畜牧兽医,2026,(8):29-36,8.基金项目
辽宁省自然科学基金面上项目(2025-MS-245、2025-MSLH-267) （2025-MS-245、2025-MSLH-267）
锦州医科大学-博泽青年科技人才托举项目(JY‐QT202303) （JY‐QT202303）
