辽宁中医杂志2026，Vol.53Issue(9)：15-19,后插2,6.DOI:10.13192/j.issn.1000-1719.2026.09.003
基于基因集富集分析阐释慢性阻塞性肺疾病有效方药抑制巨噬细胞炎症反应机制
Elucidating Anti-Inflammatory Mechanism of Traditional Chinese Medicine for Chronic Obstructive Pulmonary Disease Based on Gene Set Enrichment Analysis
摘要
Abstract
Objective To explore the anti-inflammatory mechanism of regulating and tonifying lung and kidney formulae[in-cluding Bufei formula(补肺方),Bufei Jianpi Formula(补肺健脾方),Bufei Yishen Formula(补肺益肾方),Yiqi Zishen Formu-la(益气滋肾方)]for chronic obstructive pulmonary disease.Methods Macrophage inflammatory model was induced by lipopo-lysaccharide(LPS),and then RNA-seq analysis was performed.Macrophage-inflammatory related pathways were obtained using gene set enrichment analysis(GSEA),and normalized enrichment score(NES)was used to identify pathways that were re-versed by traditional Chinese medicine(TCM)treatment.Results Compared with those in the normal group,244 significantly dis-turbed pathways(FDR＜0.05)were identified in the macrophage-inflammatory group,which were mainly related with inflamma-tion,immunity and metabolism.More than 50％of the above identified pathways could be significantly reversed by Dilong(Pheretima),Yinyanghuo(Epimedii Folium),Houpo(Magnoliae Officinalis Cortex)and Renshen(Ginseng Radix et Rhizoma).The NES of Bufei Yishen Formula was-1377.23,the NES of Yiqi Zishen Formula was-1250.01,the NES of Bufei Jianpi For-mula was-1041.56,and the NES of Bufei Formula was-720.02.Transforming growth factor beta production pathways were re-versed in most TCM-treated groups.Conclusion The intensity of regulating and tonifying lung and kidney formulae that reversed macrophage-inflammatory related pathways was in order of Bufei Yishen Formula,Yiqi Zishen Formula,Bufei Jianpi Formula and Bufei Formula.Dilong(Pheretima),Yinyanghuo(Epimedii Folium),Houpo(Magnoliae Officinalis Cortex)and Renshen(Ginseng Radix et Rhizoma)are the hub Chinese medicines for inhibiting the inflammatory response.Transforming growth factor beta pro-duction might be the important pathways of these formulae for inhibiting macrophage-inflammation.关键词
慢性阻塞性肺疾病/调补肺肾系列方/巨噬细胞/炎症反应/基因集富集分析Key words
chronic obstructive pulmonary disease/regulating and tonifying lung and kidney formulae/macrophage/inflamma-tion/gene set enrichment analysis分类
医药卫生引用本文复制引用
关庆洲,赵鹏,李康沉,彭岩,郭弘妍,李建生..基于基因集富集分析阐释慢性阻塞性肺疾病有效方药抑制巨噬细胞炎症反应机制[J].辽宁中医杂志,2026,53(9):15-19,后插2,6.基金项目
国家中医药传承与创新"百千万"人才工程—岐黄工程首席科学家(国中医药人教函[2021]7号) （国中医药人教函[2021]7号）
国家自然科学基金面上项目(81973822) （81973822）
博士后创新人才支持计划项目(BX20200115) （BX20200115）
国家自然科学基金青年项目(82305018) （82305018）
