西南医科大学学报2026，Vol.49Issue(5)：597-605,9.DOI:10.3969/j.issn.2096-3351.2026.05.006
PLGA载IR780光热-光动力纳米体系的构建及其体外抗肿瘤作用
Construction of PLGA-Loaded IR780 Nanoplatform for Combined Photothermal-Photodynamic Therapy and Its in Vitro Antitumor Effects
摘要
Abstract
Objective The photothermal-photodynamic nano-system(IR780-PLGA-NPs)carrying IR780 was prepared using poly(lactic-co-glycolic acid)(PLGA).The formulation was optimized through single-factor experiments,and the physicochemical properties and in vitro photothermal and photodynamic performances of the nanoparticles were investigated.The inhibitory effect of IR780-PLGA-NPs on breast cancer cells was also studied.Methods IR780-PLGA-NPs were prepared using the ultrasonic emulsifi-cation method.The one-factor-at-a-time approach was used to optimize the formula and investigate the effects of the drug-to-polymer ratio,PLGA concentration,surfactant concentration,and oil-to-water ratio on nanoparticle size,Zeta potential,polydispersity index(PDI),drug loading efficiency,and encapsulation efficiency.The photothermal and photodynamic properties of the nanoparticles were tested under near-infrared laser irradiation.The antitumor effects of the nanoparticles against 4T1 and MCF-7 tumor cells were studied at different concentrations in vitro and their dark toxicity to normal hepatocytes(AML-12 cells)was also evaluated.In addition,the cellular uptake behavior of nanoparticles by tumor cells was also investigated via fluorescence microscopy to elucidate the underlying antitumor mechanism.Results The optimal formulation for preparing IR780-PLGA-NPs was determined as follows:sodium cholate concentration of 15 mg/mL,oil-to-water ratio of 6.4:10.0,PLGA concentration of 18.75 mg/mL,and drug-to-polymer ratio of 1:24.The prepared IR780-PLGA-NPs exhibited an average particle size of(135.33±0.49)nm,PDI of(0.255±0.010),Zeta potential of(-10.67±0.09)mV,drug loading efficiency of(3.43±0.01)％,and encapsulation efficiency of(85.79±0.20)％.In vitro experiments demonstrated that IR780-PLGA-NPs possessed superior photothermal and photodynamic effects compared to free IR780,leading to significantly enhanced inhibition of breast cancer cell growth.Moreover,IR780-PLGA-NPs can reduce the dark cytotoxicity of IR780 to normal hepatocytes.The enhanced antitumor effects of IR780-PLGA-NPs might be related to the higher cellular uptake by tumor cells and improved photothermal stability.Conclusion The PLGA-IR780 photothermal-photodynamic nanosystem prepared in this study can effectively inhibit the growth of breast cancer cells and reduce the toxicity to normal cells,laying the foundation for it to be used as an anti-breast cancer phototherapy agent.关键词
IR780/PLGA纳米粒/乳腺癌/光热/光动力Key words
IR780/PLGA nanoparticles/Breast cancer/Photothermal therapy/Photodynamic therapy分类
医药卫生引用本文复制引用
杨佳丽,甘海霞,钟鑫童,范清泽,罗宏丽..PLGA载IR780光热-光动力纳米体系的构建及其体外抗肿瘤作用[J].西南医科大学学报,2026,49(5):597-605,9.基金项目
国家自然科学基金(22208269) （22208269）
四川省大学生创新训练计划项目(S202510632262) （S202510632262）
