内蒙古农业大学学报(自然科学版)2026,Vol.47Issue(4):72-78,7.DOI:10.16853/j.cnki.1009-3575.2026.04.009
马铃薯产量和氮代谢关键酶对肥菌配施的响应
Response of Potato Yield and Key Enzymes of Nitrogen Metabolism to Combined Application of Fertilizer and Microbial Inoculant
摘要
Abstract
Both stable fertilizers and biological bacterial fertilizers have a great impact on crop yield and quality,but the physiologi-cal effects of combination application of fertilizer and microbial inoculant on key enzymes of nitrogen metabolism in potatoes are un-clear.This study selected the potato variety'Favorita'to investigate the effects of combined fertilizer and microbial inoculant appli-cation on potato yield,as well as the activities and transcription levels of nitrate reductase(NR),nitrite reductase(NiR),and as-paraginase(AS)in leaves.The results revealed that,compared to the control group,the combination application of fertilizer and mi-crobial inoculant could increase the yield of potato by about 160%.The activities of NR and NiR in potato leaves significantly in-creased under this treatment,although their activities showed dynamic changes throughout the growth period.Among different bacte-rial fertilizer treatments,the transcription levels of StNR,StNiR and StAS in potato leaves during the middle and late stages of growth were significantly affected,indicating that the combination application of fertilizer and microbial inoculant could effectively optimize the nitrogen absorption and assimilation efficiency of potato.Notably,the JW380 treatment(i.e.,stable fertilizer+Bp.16 and Sn.188 inoculant mixed in a ratio of 1∶1)demonstrated superior effects compared to single applications of chemical fertilizer or inoc-ulant treatment in improving the activities of key enzymes in nitrogen metabolism and the transcription level of related genes.These findings provide an important theoretical basis for in-depth analysis and reasonable application of the interaction mechanism of combi-nation application of fertilizer and microbial inoculant in potato production.关键词
马铃薯/产量/氮代谢关键酶/基因表达Key words
Potato/Yield/Key enzymes of nitrogen metabolism/Gene expression分类
农业科技引用本文复制引用
盖瑶瑶,王雪瑶,薛慧玲,张岚,郭晓晴,杨晶,张子义..马铃薯产量和氮代谢关键酶对肥菌配施的响应[J].内蒙古农业大学学报(自然科学版),2026,47(4):72-78,7.基金项目
内蒙古自治区重点研发和成果转化计划项目(2023YFDZ0003,2022YFHH0130) (2023YFDZ0003,2022YFHH0130)