内蒙古农业大学学报（自然科学版）2026，Vol.47Issue(4)：79-88,10.DOI:10.16853/j.cnki.1009-3575.2026.04.010
扁蓿豆MrALC的基因克隆、亚细胞定位及表达分析
Gene Cloning,Subcellular Localization and Expression Analysis of MrALC in Medicago ruthenica
摘要
Abstract
Medicago ruthenica,also known as"Biandouzi"or flower alfalfa,is a high-quality perennial leguminous forage grass.It exhibits exceptional environmental adaptability,such as drought resistance,cold tolerance,tolerance to poor soils,and trampling resistance,making it an ideal species for natural grassland improvement and artificial grassland planting.Therefore,enhancing the seed yield of Medicago ruthenica holds significant practical and ecological value for natural grassland restoration,high-yield ar-tificial pasture construction,and comprehensive grassland ecosystem management.Previous studies have shown that the alc mu-tant of Arabidopsis thaliana displays indehiscent siliques,and the ALC gene has been identified as a key gene regulating silique dehiscence in Arabidopsis thaliana.However,its function in Medicago ruthenica remains unclear.The present study aimed to in-vestigate the regulatory role of the MrALC gene in pod development of Medicago ruthenica,breed new germplasm of Medicago ruthen-ica with low pod shattering,and solve the problem of low seed yield in Medicago ruthenica.In this study,the complete coding sequence region(1 062 bp)of the MrALC cDNA was amplified from Medicago ruthenica using PCR.Bioinformatics analysis showed that this gene encodes 353 amino acids,with 28 positively charged residues and 34 negatively charged residues,a molecular weight of 38 234.28 Da,an isoelectric point(pI)of 6.06,The protein exhibited hydrophilic characteristics,as evidenced by its predicted grand average of hydropathy score of-0.667.With an instability index of 76.03,it was classified as an unstable hydro-philic acidic protein.The protein harbors a canonical bHLH protein structural domain,comprising 17.1%alpha helix and 82.32%random coils.Subcellular localization results demonstrated nuclear localization of MrALC.The results of expression analysis in different tissues and organs showed that the expression level of MrALC in different tissues was pod>flower>stem>leaf>root,and the expression levels of pod and flower were significantly different from those of root,stem and leaf(P<0.01).The expression analysis of MrALC in different development stages of pods showed that the expression level of MrALC in different development stages of pods was 12 day after flowering(DAF)>16 DAF>8 DAF>20 DAF,and the expression levels of pods at 12 DAF and 16 DAF were significantly different from those at 8 DAF and 20 DAF(P<0.01).The above results provided a research basis for further revealing the molecular mechanism by which the MrALC gene regulates pod development in Medicago ruthenica,and also offered a scientific foundation for creating new Medicago ruthenica germplasm with low pod shattering using molecular breeding techniques.关键词
扁蓿豆/基因克隆/MrALC/亚细胞定位/表达分析Key words
Medicago ruthenica/Gene cloning/MrALC/Subcellular localization/Expression analysis分类
农业科技引用本文复制引用
雷慧,郭慧琴,于佳..扁蓿豆MrALC的基因克隆、亚细胞定位及表达分析[J].内蒙古农业大学学报（自然科学版）,2026,47(4):79-88,10.基金项目
内蒙古自治区种业科技创新重大示范工程"揭榜挂帅"项目(2022JBGS0040) （2022JBGS0040）
