宁夏农林科技2026，Vol.67Issue(8)：1-7,7.DOI:10.3969/j.issn.1002-204x.2026.08.001
BSA技术方法的研究进展
Research Progress of BSA Technology Methods
摘要
Abstract
Bulked segregant analysis(BSA)is an extreme phenotyping strategy based on genetic segregating populations.It screens individuals with extreme phenotypes in the offspring of the parental populations for pooling,compares the genetic polymorphisms of different extreme pooled samples,and combines the phenotypic information to achieve the preliminary localization of target genes or quantitative trait loci(QTL).The BSA method is suitable for the study of traits less affected by the environment and has the advantages of being fast and efficient.The development history of BSA is reviewed,and a comprehensive overview is provided around the sequencing strategy of BSA,different population types,commonly used mapping algorithms,methods for determining relevant region thresholds,and the key factors affecting the positioning accuracy of BSA.This provides theoretical references and method guidance for the application of BSA in crop breeding research.关键词
BSA/基因挖掘/下一代测序/QTLKey words
BSA/Gene mining/Next-generation sequencing/QTL分类
生物科学引用本文复制引用
施安,沈文娟,王辉辉,张鑫,李翾晟,王锦..BSA技术方法的研究进展[J].宁夏农林科技,2026,67(8):1-7,7.基金项目
宁夏回族自治区重点研发计划项目(2024BBF01010). （2024BBF01010）
