农业环境科学学报2026，Vol.45Issue(8)：1927-1939,13.DOI:10.11654/jaes.2025-0828
邻苯二甲酸二(2-乙基己基)酯胁迫下不同水稻品种生理与代谢响应
Physiological and metabolic responses of different rice varieties to di(2-ethylhexyl)phthalate stress
摘要
Abstract
To elucidate the physiological toxicity and underlying metabolic regulatory mechanisms of di(2-ethylhexyl)phthalate(DEHP)in different rice genotypes,hydroponic experiments were conducted to assess the effects of DEHP exposure on seedlings of an inbred indica cultivar(NX24)and a three-line hybrid indica cultivar(TYHZ).Untargeted metabolomics was employed to characterize genotype-specific metabolic response profiles.DEHP exposure markedly inhibited root growth in both genotypes(maximum reduction of 36.94%)and suppressed biomass accumulation.DEHP also induced genotype-specific oxidative stress responses:hydrogen peroxide content increased significantly in NX24(by 43.61%),whereas malondialdehyde levels were significantly elevated in TYHZ(by 36.69%).Distinct antioxidant systems were mobilized to counteract oxidative damage:superoxide dismutase activity in NX24 was strongly induced(an increase of 90.06%),while catalase activity in TYHZ was markedly enhanced(by 447.70%).Metabolomic pathway analysis revealed that under DEHP stress,NX24 primarily activated metabolic routes associated with cellular homeostasis,reactive oxygen species scavenging,and energy metabolism,notably sphingolipid metabolism,plant hormone signal transduction,flavonoid biosynthesis,and pantothenate and CoA biosynthesis.In contrast,TYHZ responded primarily by upregulating pathways implicated in reinforcing physical barriers(cutin,suberin,and wax biosynthesis)and maintaining membrane integrity and signaling(linoleic acid metabolism).Collectively,DEHP exerts substantial phytotoxic effects on rice seedlings,with divergent coping strategies observed between genotypes.NX24 relies predominantly on modulation of secondary metabolism and hormone signaling,whereas TYHZ preferentially enhances its physical barriers and membrane integrity.关键词
邻苯二甲酸二(2-乙基己基)酯/水稻/氧化应激/代谢响应/基因型差异Key words
di(2-ethylhexyl)phthalate/rice/oxidative stress/metabolic response/genotype differences分类
农业科技引用本文复制引用
陈璘涵,谢宁,王辉,郑飞予,姜美玲,罗斯,杨洋,吴伟健,邓潇..邻苯二甲酸二(2-乙基己基)酯胁迫下不同水稻品种生理与代谢响应[J].农业环境科学学报,2026,45(8):1927-1939,13.基金项目
湖南省自然科学基金项目(2025JJ50193) （2025JJ50193）
生态环境部重金属污染监测重点实验室开放基金项目(KLMHM202430) Natural Science Foundation of Hunan Province(2025JJ50193) （KLMHM202430）
State Environmental Protection Key Laboratory of Monitoring for Heavy Metal Pollutants(KLMHM202430) （KLMHM202430）
