农业环境科学学报2026，Vol.45Issue(8)：1952-1969,18.DOI:10.11654/jaes.2025-0937
固定化菌剂驱动下PAHs污染农田土壤固碳消污的效应及机制
Effects and mechanisms of carbon sequestration and pollution remediation in PAHs-contaminated farmland soils driven by immobilized bacterial agents
摘要
Abstract
To address the dual challenges of soil polycyclic aromatic hydrocarbons(PAHs)contamination and climate change,this study developed a biochar-immobilized microbial agent(BIM)with carbon sequestration and PAH removal capabilities.Pot experiments were conducted to investigate the optimal application strategy for BIM and elucidate its underlying mechanisms.Results indicated that BIM at a 1%dosage(BIM1%)demonstrated optimal carbon sequestration and PAHs removal efficacy,enhancing soil PAHs removal rates by 138.70%±16.42%and reducing PAH accumulation in lettuce by 76.86%±2.49%.Concurrently,cumulative CO2 emissions were significantly reduced,while total carbon(TC)and soil organic carbon(SOC)content increased significantly(P<0.05).Based on the Random Forest(RF)model,we found that BIM's contribution to carbon sequestration and PAHs removal primarily depended on changes in microbial properties.BIM1%treatment significantly increased the abundance of carbon-fixing microorganisms and PAH-degrading microorganisms in soil,along with the activity of carbon sequestration-associated enzymes,representing key influencing factors.The robustness of co-occurrence networks and cooperation among microbial species also exerted positive effects on PAHs removal and carbon sequestration.Furthermore,the significant enrichment of carbon-fixing microorganisms(ASV_64 and ASV_145)and PAH-degrading microorganisms(ASV_264 and ASV_2943)in the BIM1%treatment enhanced both carbon sequestration and PAH removal efficiency.The BIM prepared in this study holds promise for simultaneously achieving PAH removal and CO2 sequestration,offering a potential solution for ensuring agricultural production safety and mitigating climate change.关键词
多环芳烃/固碳/固定化菌剂/微生物群落/共现网络Key words
polycyclic aromatic hydrocarbons/carbon sequestration/immobilized microbial agents/microbial communities/co-occurrence networks分类
资源环境引用本文复制引用
张友爱,汤磊,凌婉婷,Tatiana Minkina,高彦征..固定化菌剂驱动下PAHs污染农田土壤固碳消污的效应及机制[J].农业环境科学学报,2026,45(8):1952-1969,18.基金项目
国家重点研发计划项目(2023YFE0110800) （2023YFE0110800）
国家自然科学基金项目(42261144738,U22A20590,42430703) National Key Research and Development Program of China(2023YFE0110800) （42261144738,U22A20590,42430703）
National Natural Science Foundation of China(42261144738,U22A20590,42430703) （42261144738,U22A20590,42430703）
