农业环境科学学报2026，Vol.45Issue(8)：1981-1994,14.DOI:10.11654/jaes.2025-0723
土壤-蒜苗系统中抗生素抗性基因分布特征与迁移机制
Distributions characteristics and transport drivers of antibiotic resistance genes in the soil-garlic seedling system
摘要
Abstract
To investigate the pollution distribution characteristics,migration patterns,and driving factors of antibiotic resistance genes(ARGs)in a soil-garlic seedling(Allium sativum L.)system.Soil,root,and leaf samples were collected from garlic seedlings at four greenhouse cultivation bases.High-throughput quantitative PCR(HT-qPCR)was employed to quantify the abundance of ARGs and integrons.Soil physicochemical properties were determined using standard chemical analysis.Microbial community structure was characterized via 16S rRNA gene high-throughput sequencing.Spearman correlation analysis,Adonis tests,and Network analysis were employed to elucidate the associations between ARGs and environmental factors.The results showed that a total of 186 distinct ARGs were detected across the soil-garlic seedling system.The relative abundance of ARGs exhibited a gradient attenuation pattern:soil>root>leaf.The dominant ARGs also shifted with plant compartment:mexF dominated in soil,mexF or tetQ dominated in root,and tetQ dominated in leaf.Correlation analysis revealed that soil pH,soil organic matter(SOM),and total nitrogen(TN)were significantly negatively correlated with multiple ARGs(P<0.01),while ammonium nitrogen(NH+4-N)significantly promoted ARG enrichment(P<0.01).The intI-1(clinic)and cIntI-1(class 1)were only detected in soil and root.They showed highly significant positive correlations with mexF,sul1,tetG-01,and tetQ in soil(P<0.01),but only with tetQ in root(P<0.01).Efflux pump-mediated ARGs(mexF,tetG-01,floR and oprJ)showed significant correlations(P<0.05)with various dominant bacterial families and functional genes,indicating broad host adaptability and potential for horizontal transfer.Endophytic bacterial families(norank_o_Chloroplast,Mitochondria)in roots and leaves were significantly negatively correlated with ARGs(P<0.05),whereas most dominant soil bacterial families showed significant positive correlations with ARGs(P<0.05).Our results indicated that soil serves as the primary reservoir for ARGs.The root and leaf tissues of garlic seedlings were observed to form a biological barrier to ARG migration via selective enrichment.Soil physicochemical properties(notably NH+4-N),horizontal gene transfer mediated by integrons,and specific soil microbial communities significantly influence(P<0.05)the distribution and migration of ARGs within the soil-garlic seedling system.关键词
抗生素抗性基因/土壤-蒜苗系统/污染分布特征/迁移规律/微生物群落Key words
antibiotic resistance genes(ARGs)/soil-garlic seedling system/pollution distribution/migration pattern/microbial community分类
农业科技引用本文复制引用
黄薇,刘洋,饶秋华,刘兰英,李玥仁,罗土炎..土壤-蒜苗系统中抗生素抗性基因分布特征与迁移机制[J].农业环境科学学报,2026,45(8):1981-1994,14.基金项目
福建省属公益类科研院所基本科研专项(2022R1022002) （2022R1022002）
福建省农业科学院自由探索科技创新项目(ZYTS20230010) （ZYTS20230010）
福建省农业科学院科技服务项目(2025KF20) Special Scientific Research Funds for Public Scientific Research Institution of Fujian(2022R1022002) （2025KF20）
Independent Exploration and Technological Innovation Project of Fujian Academy of Agricultural Sciences(ZYTS20230010) （ZYTS20230010）
Technology Service Project of Fujian Academy of Agricultural Sciences(2025KF20) （2025KF20）
