农业资源与环境学报2026，Vol.43Issue(5)：1200-1207,8.DOI:10.13254/j.jare.2025.0733
沼渣替代化肥对小白菜产量及N2O产生过程的影响机制
Effects of replacing chemical fertilizer with biogas residue on Pakchoi yield and N2O emission
摘要
Abstract
This study quantified the mitigation effect of substituting chemical fertilizer with biogas residue(BR)on nitrous oxide(N2O)emissions in a Pakchoi(Brassica rapa subsp.Chinensis)cultivation system,elucidating the competitive mechanisms underlying N2O production pathways mediated by microbial functional activity.A pot experiment was conducted using BR substitution at gradients of 10%(BR1),30%(BR2),and 50%(BR3)on an equal nitrogen basis,alongside a chemical fertilizer control(CK).Soil physicochemical properties,pakchoi yield,the abundance of nitrifying and denitrifying microorganisms,and nitrification/denitrification potential were measured.The results showed that,the yield in the BR1 treatment did not differ significantly from CK,whereas yields in BR2 and BR3 decreased significantly by 31.4%and 48.8%,respectively(P<0.05).All BR treatments significantly reduced both nitrification and denitrification potential,with the most substantial reduction observed in BR1.BR substitution primarily reduced the N2O production potential by diminishing the abundance of bacterial nitrifying and denitrifying functional groups and enhancing the N2O reduction.Conversely,it promoted N2O production via fungal heterotrophic nitrification and denitrification.Pathway analysis indicated that bacteria dominated N2O production in the pakchoi system.Notably,the BR1 treatment shifted the dominant source of N2O production from bacteria to fungi,indicating a transition in the microbial pathway.These findings demonstrate that 10%BR substitution represents the optimal rate for synergistically reducing chemical fertilizer application and mitigating N2O emissions.关键词
沼渣替代/氧化亚氮/功能基因/真菌异养硝化/硝化反硝化潜势Key words
biogas residue substitution/N2O/functional gene/fungal heterotrophic nitrification/nitrification and denitrification potential引用本文复制引用
肖辉,张瑜煊,张非凡,宋玉凤,钟磊,刘佳,张杰,陈冠益..沼渣替代化肥对小白菜产量及N2O产生过程的影响机制[J].农业资源与环境学报,2026,43(5):1200-1207,8.基金项目
国家重点研发计划项目(2023YFD1902603) （2023YFD1902603）
天津科技计划项目(24YDTPJC00310) （24YDTPJC00310）
天津市农业产学研用"揭榜挂帅"项目(GBGG202417) （GBGG202417）
